摘要
随着人类社会工业化进程的加快,重金属镉(Cd)污染对海洋生物的毒害作用突显。为了探索Cd胁迫下长茎葡萄蕨藻生理调控的分子响应机制,本文采用RNA-Seq技术对镉胁迫(Hcd^(2+))藻体组织转录组进行研究。结果表明:与对照相比,Hcd^(2+)组共筛选出差异表达基因(DEGs)702个,其中,257个基因上调表达,445个基因下调表达。对获得的DEGs进行功能注释及富集分析,发现长茎葡萄蕨藻的多种生物学功能受到Cd^(2+)胁迫的影响,最终表现为抑制生长。GO富集分析发现,长茎葡萄蕨藻体内氧自由基的产生与清除出现失衡,并产生了DNA损伤等氧化伤害。KEGG富集分析发现,长茎葡萄蕨藻中多条与光合作用相关途径受到抑制,表明Cd^(2+)胁迫导致长茎葡萄蕨藻的光合反应紊乱。
With the acceleration of industrialization,the toxic effect of heavy metal cadmium(Cd) pollution has become prominent.In order to explore the molecular mechanism of the physiological regulation of Caulerpa lentillifera under Cd stress,we analyzed the transcriptome of Cd-stressed(Hcd^(2+)) algae tissues using RNA-Seq.A total of 702 differentially expressed genes(DEGs)were screened between the control and Hcd^(2+) groups,out of which 257 genes were up-regulated and 445 genes were down-regulated in the Hcd^(2+) group.We conducted functional annotation and enrichment analysis of the obtained DEGs.The results showed that various biological functions of C.lentillifera were affected under Cd^(2+)stress,which eventually showed growth inhibition.Results of GO enrichment analysis showed that the production and removal of reactive oxygen species(ROS)in C.lentillifera were out of balance and caused oxidative damage such as DNA damage.Results of KEGG enrichment analysis showed that many photosynthesis-related pathways were inhibited,indicating that Cd^(2+)stress led to disorder of photosynthetic reaction of C.lentillifera.
作者
庞美霞
黄志立
刘小龙
唐勇军
李晓东
金刚
PANG Mei-xia;HUANG Zhi-li;LIU Xiao-long;TANG Yong-jun;LI Xiao-dong;TIN Gang(Postdoctoral Innovation Practice Base,Shenzhen Polytechnic,Shenzhen 518055,Guangdong,China;School of Applied Chemistry and Biological Technology,Shenzhen Polytechnic,Shenzhen 518055,Guangdong,China)
出处
《应用生态学报》
CAS
CSCD
北大核心
2021年第12期4447-4456,共10页
Chinese Journal of Applied Ecology
基金
深圳职业技术学院博士后启动基金项目(6019330003K)
深圳市科技项目(JCYJ20170818140317993)资助。
关键词
长茎葡萄蕨藻
镉胁迫
分子机制
转录组
Caulerpa lentillifera
cadmium stress
molecular mechanism
transcriptome