基于贝尔曼漏斗法的土壤线虫最优分离时长探究

Study on Optimal Extraction Duration of Soil-Nematode Based on Baermann Funnel Method

贝尔曼漏斗法是土壤线虫分离中较为常用的方法,但现有研究使用的分离时长差异较大,缺乏统一标准。为探究其最优分离时长,该研究利用贝尔曼漏斗法分离土壤线虫,连续72 h进行计数,分别计算12、24、36、48、60、72 h的累积分离率。结果显示,就分离种类而言24 h为峰值,与48 h比较差异无统计学意义(21.00±2.54 vs. 21.00±1.00,P=0.817);就分离数量而言36h是最适时长,累计分离率达99.63%。实验共分离出线虫24 777头,属2纲6目21科35属,涵盖了较多的线虫类群,具有普适性。综合分离种类和数量,推荐贝尔曼漏斗法的分离时长为36 h。

The Baermann funnel method is common in soil-nematode extraction.However,it has been found that the extraction durations in studies varies greatly,and there is a lack of agreed standard.To explore the optimal extraction duration,the Baermann funnel method was used to extract soil-nematode for 72 h,and the cumulative separation rates were calculated for 12,24,36,48,60 and 72 h respectively.The results showed that in terms of the separation of taxa,24 h was the peak value,and it has no difference from that of 48 h(21.00±2.54 vs.21.00±1.00,P=0.817);in terms of the separation of numbers,36 h was the most appropriate time,and the cumulative separation rate was 99.63%.A total of 24777 nematodes were isolated in the experiment,belonging to 2 classes,6 orders,21 families and 35 genera,covering a large number of nematode groups and having universal applicability.Considering the taxa and numbers,the recommended separation time of the Bellman funnel method is 36 h.