小麦8亲本高世代互交群体的创制与应用

Creation and Application of an Eight-Parent Advanced Generation Intercross Population of Wheat

多亲本高世代互交(multiparent advanced generation intercross,MAGIC)群体是新一代的遗传群体,具有重组充分和遗传多样性丰富的特点。目前,弱筋小麦生产存在品质不稳定、优质弱筋原粮不能满足饼干等食品企业需求的状况,亟需培育高产优质弱筋小麦新品种来解决上述问题。针对这个问题,创制了一个含有3123株系的小麦8亲本MAGIC群体。通过田间农艺性状观测、种子形态指标测量、千粒重相关分子标记检测和籽粒品质测定,得到以下结果:1)MAGIC群体主要农艺性状变异范围大,是高效的遗传研究群体;2)种子的长度、宽度、直径和周长与千粒重无显著相关性(R^(2)<0.3);3)TaGASR7-A1null等位基因与低粒重相关,而TaGS-D1null等位基因与高粒重相关;4)获得高产低筋小麦候选株系20份。

Multiparent advanced generation intercross(MAGIC)populations are one of the new generation of crop genetic population with high genetic recombination and diversity.At present,the quality of the elite weak-gluten wheat is unstable,and there are gaps between yield of elite weak-gluten wheat and raw material demands of biscuit manufacturers.Hence,it is urgent to cultivate new wheat varieties with stable quality(elite weak-gluten)and high yield.In this study,an eight-parent advanced generation intercross wheat population,which had 3123 inbred lines were created.The traits such as agronomic character,seed phenotype,the quality of the seed of inbred lines were detected,the polymorphism of thousand-kernel-weight(TKW)related gene was also examined.The results showed that:1)The MAGIC population is an efficient genetic mapping population because of its wide variation of main agronomic traits.2)The correlation between TKW and seed length,width,diameter and circumference is low(R^(2)<0.3).3)Molecular markers revealed that TaGASR7-A1null allele is associated with low TKW,while TaGS-D1null allele relate to high TKW.4)Twenty candidate wheat lines with low gluten and high TKW were obtained.