枸杞多糖对肝内胆汁淤积大鼠血液指标和肝组织形态的影响

Effects of Lycium barbarum polysaccharides on blood indexes and liver tissue morphology in rats with intrahepatic cholestasis

目的研究枸杞多糖对肝内胆汁淤积大鼠血液指标和肝组织形态的影响。方法雄性SD大鼠50只,随机分为正常对照组,模型对照组和枸杞多糖低、中、高剂量组。模型对照组及枸杞多糖各剂量组大鼠于实验开始当天给予α-奈异硫氰酸脂(ANIT)60 mg/kg灌胃,以后每隔3天一次;正常对照组以色拉油代替ANIT。第3天起,各剂量组大鼠分别给予40、150、600 mg/kg枸杞多糖,模型对照组大鼠给予等体积蒸馏水。4周后测定大鼠血液和尿液指标,观察肝组织形态。结果实验第3天起,模型对照组和各剂量组大鼠活动减少,尿液颜色均发生改变,呈深黄色;正常对照组大鼠未见异常。模型对照组大鼠总胆红素、直接胆红素、总胆汁酸(TBA)、碱性磷酸酶(ALP)、γ-谷氨酰转移酶(γ-GGT)、胆固醇、谷丙转氨酶(ALT)、谷草转氨酶(AST)、白细胞(WBC)、粒细胞百分比、尿胆红素、尿胆汁酸、肝重量、肝体比均高于正常对照组,红细胞和淋巴细胞百分比低于正常对照组(均P<0.05);肝组织形态发生病理性改变。枸杞多糖高剂量组大鼠血TBA、ALP、γ-GGT、ALT、AST、WBC和肝体比低于模型对照组(均P<0.05);肝脏炎细胞浸润,胆管增生、扩张,以及肝细胞变性、坏死等病理性改变均减轻。结论600 mg/kg剂量枸杞多糖可改善肝内胆汁淤积大鼠的血液指标和肝组织病理性改变。抑制炎症反应、减轻氧化应激损伤可能是枸杞多糖缓解胆汁淤积性肝损伤的重要机制。

Objective To study the effects of Lycium barbarum polysaccharides(LBP)on blood indexes and liver tissue morphology in rats with intrahepatic cholestasis.Methods Sprague-Dawley rats were randomly divided into the control group,the model group,and LBP low,medium and high dose group.The rats in the model group and LBP dose groups were given 60 mg/kg alpha-naphthylisothiocyanate(ANIT)by gavage every three days of the experiment,and the rats in the control group were given salad oil instead of ANIT.From the third day,the rats in each dose group were given 40,150 and 600 mg/kg LBP,and the rats in the model group were given distilled water.After four weeks,the blood and urine indexes were measured,and the morphological changes of liver tissue were observed.Results From the third day of the experiment,the activity of rats in the model group and LBP dose groups decreased,and the color of urine changed to dark yellow.There was no abnormality in the group.In the model group,the levels of serum total bilirubin,direct bilirubin,total bile acid(TBA),alkaline phosphatase(ALP),γ-glutamyltransferase(γ-GGT),cholesterol,alanine aminotransferase(ALT),aspartate aminotransferase(AST),white blood cell(WBC),percentage of granulocyte,urinary bilirubin,urinary bile acid,liver mass and liver to body ratio were higher than those in the control group,while red blood cell and percentage of lymphocyte were lower than those in the control group(all P<0.05).Pathological changes of liver tissue were observed.The levels of serum TBA,ALP,γ-GGT,ALT,AST,WBC and liver to body ratio in LBP high dose group were lower than those in the model group(all P<0.05).The infiltration of inflammatory cells,proliferation and expansion of bile duct,degeneration and necrosis of liver cells were alleviated.Conclusions LBP can improve the blood indexes and pathological changes of liver tissue in rats with intrahepatic cholestasis at the dosage of 600 mg/kg.Inhibition of inflammatory response and reduction of oxidative stress injury may be the mechanism for alleviating cholestatic liver injury.