阿魏酸通过调控线粒体凋亡抑制宫颈癌HeLa细胞侵袭迁移功能

Ferulic acid inhibits the invasion and migration of cervical cancer HeLa cells by regulating mitochondrial apoptosis in vitro

目的:探讨阿魏酸(FA)对宫颈癌HeLa细胞株凋亡调控作用及其可能机制。方法:体外培养HeLa细胞,将其分为control组和FA处理组(1、2和4 mmol/L)。细胞计数试剂盒8(CCK-8)和划痕实验检测细胞增殖和迁移能力,Transwell实验检测细胞的侵袭能力。实时定量聚合酶链反应(RT-q PCR)检测不同浓度FA处理后HeLa细胞凋亡相关基因Bcl-2、Bax及Cleaved-caspase-3的表达,免疫荧光检测HeLa细胞中线粒体的表达数量及状态。结果:RT-q PCR结果显示,与control组相比,随着FA浓度增高,HeLa细胞中Bcl-2基因表达逐渐降低,而Bax和Cleaved-caspase-3基因表达逐渐增强(P<0.05)。免疫荧光示,与control组相比,HeLa细胞中的线粒体含量逐渐减少且功能逐渐减弱(P<0.05)。Transwell以及划痕实验结果显示,与control组相比,不同浓度FA可使HeLa细胞的侵袭细胞数以及迁移细胞数减少且呈剂量依赖性(P<0.05)。结论:FA通过Bcl-2家族蛋白途径抑制HeLa细胞的增殖、侵袭及迁移,诱导线粒体介导的细胞凋亡。

Objective:To study the effect of Ferulic Acid (FA) on cervical cancer (HeLa) cell lines and its possible mechanism.Methods:HeLa cells were cultured in vitro and divided into the control group and FA treatment group (1,2 and 4 mmol/L).CCK-8 and scratch test detected cell proliferation and migration ability.Transwell test evaluated cell invasion ability.Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of apoptosis-related genes Bcl-2,Bax and Cleaved-caspase-3 in HeLa cells after treatment with different concentrations of FA,and immunofluorescence was used to detect the expression and status of mitochondria in HeLa cells.Results:RT-qPCR results showed that the expression of Bcl-2gene in HeLa cells gradually decreased,while the expression of Bax and Cleaved-caspase-3 genes gradually increased in a dose-dependent manner,with statistical difference (P<0.05),compared with the control group.Immunofluorescence showed that the content of mitochondria in HeLa cells gradually decreased and the function gradually weakened with statistical difference (P<0.05),compared with the control group.The results of Transwell and scratch experiments showed that different concentrations of FA reduced the number of invaded cells and migrated cells of HeLa cells in a dose-dependent manner with significant differences (P<0.05),compared with the control group.Conclusion:FA inhibits the proliferation,invasion and migration of HeLa cells through the Bcl-2 family protein pathway,and induces mitochondrial-mediated apoptosis.