蒲公英根活性组分体外抗菌及抗炎作用

In vitro antibacterial and anti-inflammatory activity of active components from dandelion root

以市售蒲公英根为研究对象,采用乙醇提取、大孔树脂柱分离和活性跟踪相结合的方法优选蒲公英根抗菌抗炎活性组分,通过倍半稀释法测定蒲公英根不同组分对人、畜禽等9种常见病原菌的抑菌作用,通过脂多糖诱导的小鼠巨噬细胞RAW264.7炎症细胞模型评价各组分的抗炎活性。结果表明,蒲公英根抗菌抗炎活性组分的分离工艺为:70%乙醇回流提取后,用D101大孔树脂柱分离,其中,水洗脱之后的70%(体积分数)乙醇洗脱部位为蒲公英根的抗菌抗炎活性部位,即蒲公英根抗菌抗炎活性组分;该组分黄酮含量相对较高,且对9种供试菌具有较强的广谱抗菌性,大多数最小抑菌质量浓度为1.95 mg/mL,最小杀菌质量浓度均为31.25 mg/mL,对脂多糖诱导的小鼠巨噬细胞RAW264.7的炎症因子IL-6、IL-1β和TNF-α的浓度和转录水平均有显著抑制作用(P<0.05)。优选的蒲公英根抗菌抗炎活性组分分离工艺简便、高效,而且具有较强的广谱抗菌作用和良好的抗炎作用。

Ethanol extraction,macroporous resin column separation and activity tracking were used for the selection of antibacterial and anti-inflammatory components from dandelion root.The antibacterial and anti-inflammatory activities in vitro against nine common pathogens were determined by the half dilution method,and the anti-inflammatory activity in vitro was assayed by the lipopolysaccharides(LPS)-induced macrophagocyte RAW 264.7.The results showed that the antibacterial and anti-inflammatory active components from dandelion root were separated by D101 macroporous resin column after 70%ethanol reflux extraction.The results showed that 70%ethanol elution fraction,after water elution,presented antibacterial and anti-inflammatory activities.The active components had more flavonoids and broad-spectrum antimicrobial activity against nine tested bacteria(most MIC values were 1.95 mg/mL and all MBC values were 31.25 mg/mL)and the outstanding inhibition of the concentration and transcription levels of inflammatory cytokines IL-6,IL-1βand TNF-αin LPS-induced macrophagocyte RAW 264.7(P<0.05).The antimicrobial and anti-inflammatory components from the dandelion root have simple and efficient separation technology and broad-spectrum antimicrobial activity and outstanding anti-inflammatory activity.