水提法提取荸荠多糖及其体外抗氧化活性研究

Study on water extraction of polysaccharide of Chinese water chestnut and its antioxidant characters in vitro

采用水提法提取荸荠多糖,通过单因素和正交试验优化提取参数,并对荸荠多糖进行体外抗氧化活性测定。结果表明,多糖得率最高的提取参数为:乙醇体积分数80%、料液比1∶5(g∶mL)、解析时间32 min、提取时间20 min,在此条件下荸荠多糖提取率为17.86%。体外抗氧化试验结果显示荸荠多糖对DPPH自由基和·OH有较强的清除能力,表现出较强的抗氧化活性,对DPPH自由基和·OH的清除率的半抑制浓度(half inhibitory concentration,IC_(50))值分别是0.142、0.177 mg/mL。综上所述,荸荠多糖具有良好的抗氧化活性,可作为天然抗氧化剂。

In this study,the extraction parameters of polysaccharide from Chinese Water Chestnut were optimized by single factor and orthogonal experiments,and the antioxidant activity of polysaccharide was determined.The results showed that the extraction parameters of polysaccharide yield were as follows.Ethanol concentration was 80%,solid-liquid ratio was 1∶5(g∶mL)with dissociation for 32 min and followed extraction for 20 min.Under these conditions,the yield of polysaccharide was 17.86%.The results of in vitro antioxidant test showed that polysaccharide had strong scavenging ability on DPPH and·OH,and showed strong antioxidant activity,and their half-maximal inhibitory concentration(IC_(50))value was 0.142,0.177 mg/mL.In conclusion,this study provides theoretical support for the development of high value-added water chestnut products and the utilization of water chestnut extract in health care products.