果袋类型对‘紫金红霞’葡萄果实品质及花色苷合成相关基因表达的影响

Effect of Bag Types on Fruit Quality and Anthocyanin Biosynthesis Related Genes Expression of‘Zijinhongxia’Grape Berry

以‘紫金红霞’葡萄为试验材料,在果实转色前分别进行5种不同类型果袋(白色纸袋、无纺布-白纸双层袋、绿色纸袋、蓝色纸袋、棕色纸袋)套袋处理,以不套袋为对照,测定不同发育时期葡萄果实的单果重、果粒纵横径、可溶性固形物、可滴定酸,及总花色苷含量等生理指标,并利用qRT-PCR技术分析不同发育时期花色苷合成相关基因的表达水平,探索不同类型果袋对‘紫金红霞’葡萄果实品质、花色苷含量及花色苷合成相关基因表达的影响。结果表明:(1)白色、蓝色和棕色果袋不利于成熟果实中可溶性固形物含量的升高,蓝色和棕色果袋不利于成熟果实中可滴定酸含量的降低。(2)套袋处理会使果实色泽指数显著降低,除无纺布-白纸双层袋未显著降低成熟期果皮中花色苷含量外,其他套袋处理均显著降低了果皮中总花色苷含量。(3)套袋处理对6个花色苷合成相关基因的表达主要表现为抑制作用,但无纺布-白纸双层袋对成熟期F3′H和UFGT基因的表达、白色纸袋对成熟期MYBA1、DFR和LDOX基因的表达则具有促进作用。研究发现,无纺布-白纸双层袋对成熟期果实的内在品质和果皮着色影响最小,可用于‘紫金红霞’果实套袋,其次为白色和绿色纸袋;蓝色和棕色纸袋可使葡萄果实的品质大幅降低,故不可用于实际生产中葡萄果实的套袋。

The‘Zijinhongxia’grape was used as test material,and the fruit were bagged with 5 different types of bags(white paper bag,non-woven and white paper double-ply bag,green paper bag,blue paper bag and brown paper bag)in the early stage of veraison,and with not bagging as control.In order to study the effect of different fruit bags on fruit quality,anthocyanin content and the expression of anthocyanin synthesis related genes,we determined the fruit weight,fruit vertical and horizontal diameter,total soluble solids and titratable acid of grape berries at different development stages.Meanwhile,the expression of anthocyanin synthesis related genes was also evaluated by qRT-PCR.The results showed that:(1)white,blue and brown paper bags were not favorable to the increase of total soluble solids in mature fruit,and blue and brown paper bag were not favorable to the reduction of titratable acid content in mature fruit.(2)Bagging treatment will reduce the color index of red grape significantly,and the content of anthocyanin in pericarp was reduced significantly because of bagging except that non-woven and white paper double-ply bag did not significantly influence the mature pericarp.(3)For the expression of 6 anthocyanin synthesis related genes,bagging treatment mainly behaved inhibition.However,non-woven and white paper double-ply bag promoted the up-regulated expression of F3′H and UFGT genes,and white paper bag promoted the up-regulated expression of MYBA1,DFR and LDOX genes in mature fruit.In summary,our results suggested that the effect of non-woven and white paper double-ply bag on fruit quality and fruit coloring is minimal,and this type of fruit bag can be used for fruit bagging.The second choice is white and green bag.However,because the blue and brown paper bag seriously reduces fruit quality,therefore they are not suitable for‘Zijinhongxia’fruit bagging.