瑞芬太尼通过抑制氧化应激减轻大鼠肝脏缺血再灌注损伤

Remifentanil Attenuates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress in Rats

目的探讨热休克蛋白70(HSP70)在瑞芬太尼后处理减轻大鼠肝脏缺血再灌注损伤(HIRI)中的作用。方法将24只SD大鼠随机分为4组(n=6):假手术组(S组)、HIRI组(I/R组)、瑞芬太尼组(R组)和槲皮素组(Q组)。制备大鼠HIRI模型。R组再灌注开始10 min内给予瑞芬太尼4μg/kg;Q组给予瑞芬太尼前先给予槲皮素7 mg/kg。再灌注结束后检测血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活性以及肝组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)和HSP70的含量。结果与S组比较,其余3组大鼠血清ALT、AST活性和肝组织MDA、HSP70含量均升高,而I/R组和Q组肝组织SOD活性降低、R组则升高(P<0.05)。与I/R组比较,R组大鼠血清ALT、AST活性和肝组织MDA含量降低,而肝组织SOD活性和HSP70含量升高(P<0.05)。结论瑞芬太尼后处理可减轻大鼠HIRI的急性肝损伤,抑制肝脏脂质过氧化反应,其作用机制可能与增强肝组织的HSP70表达有关。

Objective To explore the effect of heat shock protein 70(HSP70)on remifentanil-post-treatment-induced hepatic ischemia-reperfusion injury(HIRI)in rats.Methods Twenty-four Sprague-Dawley rats were randomly divided into four groups(n=6):sham-operation group(group S),HIRI group(group I/R),remifentanil group(group R)and quercetin group(group Q).Rat HIRI model was prepared.In Group R,remifentanil 4μg/kg was given at the beginning of reperfusion within 10 minutes.In group Q,quercetin 7 mg/kg was given before remifentanil treatment.The activities of serum alanine transaminase(ALT)and aspartate transaminase(AST)were measured after reperfusion,and the activity of superoxide dismutase(SOD),the content of malondialdehyde(MDA)and HSP70 in liver tissue were measured.Results Compared with group S,serum ALT and AST activity,liver MDA and HSP70 content were increased in other three groups,while SOD activity in group I/R and group Q were decreased but increased in group R(P<0.05).Compared with group I/R,serum ALT,AST activity and MDA content in group R were decreased,while liver SOD activity and HSP70 content were increased(P<0.05).Conclusion Remifentanil post-treatment can attenuate the acute liver injury induced by HIRI and inhibit the lipid peroxidation response,which is related to increasing the expression of HSP70 in liver.