抽动障碍患儿外周血淋巴细胞DRD5 mRNA的表达水平及祛风止动方对其干预作用研究

Study on expression of DRD5 mRNA in peripheral blood lymphocytes of children with tic disorder and intervention effect of Qufeng Zhidong Decoction on it

目的对抽动障碍患儿外周血淋巴细胞DRD5 mRNA表达进行检测,观察中药祛风止动方治疗对DRD5 mRNA表达的影响。方法选取100例抽动障碍患儿作为研究对象(抽动障碍组),以50名相应年龄的健康儿童青少年作为对照(正常对照组)。采用实时荧光PCR方法,分别检测2组人群及抽动障碍组的中、重度患儿在祛风止动方治疗前后的外周血淋巴细胞DRD5 mRNA表达水平。结果按照年龄段将入组儿童青少年分为学龄前期(<7岁)和学龄期(≥7岁)分别进行分析。学龄前期和学龄期抽动障碍组外周血淋巴细胞DRD5 mRNA的相对表达量均显著高于相应年龄段的正常对照组,差异有统计学意义(均P<0.05)。经祛风止动方治疗后,学龄前期、学龄期中度、重度抽动障碍组DRD5 mRNA的相对表达量均较治疗前显著降低,差异有统计学意义(均P<0.05)。结论抽动障碍的发生和发展可能与患者外周血淋巴细胞DRD5 mRNA的高表达有关,DRD5可以作为祛风止动方治疗抽动障碍的靶点。

Objective To detect the expression of DRD5 mRNA in peripheral blood lymphocytes of children with tic disorder,and observe the effect of the traditional Chinese medicine Qufeng Zhidong Decoction on the expression of DRD5 mRNA.Methods A total of 100 children with tic disorder were selected as the research objects(the tic disorder group),and 50 healthy children of the corresponding age were collected as controls(normal control group).Real-time PCR method was used to detect the expression levels of DRD5 mRNA in peripheral blood lymphocytes.The expression of DRD5 mRNA of moderate and severe patients in the tic disorder group were detected before and after the treatment of Qufeng Zhidong Decoction.Results The enrolled children were divided into preschool(<7 years old)and school-age(≥7 years old)for analysis according to their age.The relative expressions of DRD5 mRNA in peripheral blood lymphocytes in the preschool and school-age tic disorder groups were significantly higher than those of the normal control group of the corresponding age(P<0.05).After treatment with Qufeng Zhidong Decoction,the relative expressions of DRD5 mRNA in the preschool,school-age moderate tic disorder group and school-age severe tic disorder group were significantly lower than those before treatment(P<0.05).Conclusion The occurrence and development of tic disorder may be related to the high expression of DRD5 mRNA in peripheral blood lymphocytes.DRD5 can be used as the target of Qufeng Zhidong Decoction to treat tic disorder.