马齿苋降尿酸及肾脏保护作用的研究

Effect of Portulaca oleracea L. on Lowering Uric Acid and Protecting Kidney

目的:研究马齿苋降尿酸和肾脏保护作用,为临床研究提供参考。方法:将雄性ICR小鼠随机分为5组,空白组(CON)、模型组(HUA)、别嘌呤醇阳性药对照组(ALLOP,0.02 g/kg/d)、马齿苋高剂量组(PO-H,1.48 g/kg/d)、马齿苋低剂量组(PO-L,0.5 g/kg/d)。每组按相应给药剂量连续灌胃21 d,第7 d开始,除空白组外,每组腹腔注射次黄嘌呤(HX,0.3 g/kg)联合氧嗪酸钾(OAPS,0.3 g/kg)以建立高尿酸血症模型。观察小鼠血清尿酸值(SUA)、丙二醛(MDA)水平;收集肝脏检测黄嘌呤氧化酶(XOD)、超氧化物歧化酶(SOD)水平;收集肾脏检测谷胱甘肽(GSH)、谷胱甘肽过氧化酶(GSH-PX)水平;实验结束前一天收集24 h尿液检测尿尿酸(UUA)、尿肌酐(UCr)水平;对小鼠肾脏HE染色进行病理学观察。结果:与CON组比较,HUA组极显著(P<0.01)升高MDA、SUA、XOD水平,极显著(P<0.01)降低SOD、GSH、GSH-PX、UUA、UCr水平,说明高尿酸血症建模成功。与HUA组比较,PO-H组可极显著(P<0.01)降低SUA、MDA、XOD水平,极显著(P<0.01)升高SOD、GSH、GSH-PX、UCr水平;PO-L组可显著(P<0.05或P<0.01)降低SUA、MDA、XOD水平,显著(P<0.05或P<0.01)升高GSH、GSH-PX、UCr水平;HE染色结果显示PO可明显改善HUA小鼠肾组织的病理变化。结论:马齿苋提取物对高尿酸血症小鼠具有一定的降尿酸和肾保护功能。

Objective: To study the uric acid-lowering and renal protective effects of Portulaca oleracea to provide reference for clinical research. Methods: Male ICR mice were randomly divided into 5 groups. Blank group(CON), model group(HUA), allopurinol positive drug control group(ALLOP, 0.02 g/kg/d), purslane high-dose group(PO-H, 1.48 g/kg/d),Portulaca low-dose group(PO-L, 0.5 g/kg/d). Each group was given the corresponding dose for 21 d continuously, starting from the 7 th day, except for the blank group, each group was intraperitoneally injected with hypoxanthine(HX, 0.3 g/kg)combined with potassium oxazine(OAPS, 0.3 g/kg) to establish a hyperuricemia model. Observe serum uric acid(SUA)and malondialdehyde(MDA) levels in mice, collect liver to detect xanthine oxidase(XOD) and superoxide dismutase(SOD) levels, collect kidney to detect glutathione(GSH), glutathione peroxidase(GSH-PX) level. 24 hours urine was collected the day before the end of the experiment to detect urine uric acid(UUA) and urine creatinine(UCr) levels,pathological observation of mouse kidney HE staining was performed. Results: Compared with the CON group, the HUA group significantly(P<0.01) increased the levels of MDA, SUA, and XOD, and significantly(P<0.01) decreased the levels of SOD, GSH, GSH-PX, UUA, and UCr, indicating that hyperuricemia was a cause of hyperuricemia. The model was successful. Compared with HUA group, PO-H group could significantly(P<0.01) reduce SUA, MDA, XOD(levels,significantly)(P<0.01), increase SOD, GSH, GSH-PX, UCr levels. PO-L group could significantly(P<0.05 or P<0.01)reduce the levels of SUA, MDA, XOD, and significantly(P<0.05 or P<0.01) increase the levels of GSH, GSH-PX, UCr.HE staining results showed that PO could significantly improve the kidneys of HUA mice tissue pathological changes.Conclusion: Portulaca oleracea extract would have certain uric acid-lowering and renal protective functions on hyperuricemia mice.