米曲霉异源蛋白表达系统研究进展及展望

Research progress and prospects of Aspergillus oryzae heterologous protein expression system

米曲霉作为一种重要的工业微生物,在异源蛋白表达方面已有广泛应用,受限于被表达蛋白的修饰及分泌过程,目前实际生产使用的基因供体主要局限于其他真菌,尤其是丝状真菌。当外源基因来源于植物、昆虫和哺乳动物时,米曲霉所生产的异源蛋白产量及生物活性往往不尽如人意。本文综述了米曲霉作为宿主表达异源蛋白的研究进展,包括其现有的遗传操作手段及异源表达方面的应用及探索,重点介绍了应用过程中面临的挑战和解决策略,另外,对米曲霉表达异源蛋白的应用前景及发展方向进行了展望。

Aspergillus oryzae as an important industrial microorganism,in heterologous protein expression has been widely used.Limited by the modification and secretion process of the expressed protein,the gene donors currently used in actual production are mainly limited to other fungi,especially filamentous fungi.When exogenous genes were derived from plants,insects and mammals,Aspergillus oryzae produced heterologous protein yield and biological activity is often not satisfactory.This paper reviews the research progress of Aspergillus oryzae as a host for expressing heterologous proteins,including its existing genetic manipulation methods and the application and exploration of heterologous expression.In addition,the application prospects and development directions of Aspergillus oryzae expressing heterologous proteins are prospected.