基于“熵增驱动凝胶形成”理论的魔芋葡甘聚糖——表没食子儿茶素没食子酸酯拓扑微凝胶的制备及其抗氧化性研究

Preparation and Antioxidant Activity of Konjac Glucomannan:Epigallocatechin Gallate Topological Microgel Based on the Theory of Entropy-driven Gel Formation

为解决表没食子儿茶素没食子酸酯(Epigallocatechin gallate,EGCG)在实际应用中稳定性差、易被氧化的问题。基于“熵增驱动凝胶形成”理论,制备以魔芋葡甘聚糖多糖(Konjac glucomannan,KGM)微凝胶为载体的KGM-EGCG轭合物体系,通过计算机模拟和数学模型分析维系体系稳定的作用力,通过扫描电子显微镜(SEM)和红外光谱(FT-IR),对KGM-EGCG的拓扑网络的结构和性质进行系统研究,并揭示体系抗氧化稳定性可能的机理。结果表明,KGM-EGCG结合过程中,随着EGCG浓度增大,其对疏水作用的贡献会增大,氢键连接数量会增多,分子间作用力增强,存在明显的拓扑现象。通过熵增驱动形成的KGM-EGCG体系的热稳定性较好,且具有较好的O^(-)_(2)自由基和DPPH自由基清除率。KGM-EGCG微凝胶不仅具有较好的抗氧化性而且控释效果好。

To solve the problems of poor stability and easy oxidation of Epigallocatechin gallate(EGCG)in practical application.In this study,the KGM-EGCG yoke system was prepared using Konjac Glucomannan(KGM)microgel as carrier based on the theory of“entropy-driven gel formation”.The forces maintaining the stability of the system were analyzed by molecular simulation and mathematical model.The structure and properties of KGM-EGCG topological heterogeneous network were systematically studied by scanning electron microscopy(SEM)and infrared spectroscopy(FT-IR),and the possible mechanism of antioxidant stability of the system was revealed.The results showed that during the KGM-EGCG binding process,as the concentration of EGCG increases,its contribution to hydrophobic interaction,its number of hydrogen bond connections and the intermolecular forces will all increase,resulting in obvious topological isomerism.The KGM-EGCG system formed by topological isomerism driver has better thermal stability,O^(-)_(2)·scavenging force and DPPH·scavenging force.KGM-EGCG microgel has not only good antioxidant activity but also good controlled release effect.