Poloxamer188复合米托蒽醌聚D,L-乳酸-co-乙醇酸电纺纤维的表征

Characterization of Poloxamer188 Combined with Mitoxantrone-loaded Poly D,L-lactic Acid-co-glycolic Acid Electrospun Fiber

目的制备载米托蒽醌的聚D,L-乳酸-co-乙醇酸电纺纤维,考察Poloxamer188复合量和载药量对纤维形态和药物释放的影响。方法将米托蒽醌,Poloxamer188,聚D,L-乳酸-co-乙醇酸共同溶解在二氯甲烷与乙酸的共溶剂中,形成均相溶液后静电纺丝。将所得纤维采用扫描电子显微镜观察纤维形态,紫外分光光度-高效液相色谱法测定其释放量,绘制释放曲线。X-射线衍射和差示热分析法对材料复合进行表征。结果体系中Poloxamer188复合量和米托蒽醌载量的提高均有助于形成理想形态的纤维,同时也有助于药物的释放。各个纤维的突释量均较大,缓释期小于24 h,40 d平台期内的释放增量很小。随着Poloxamer188复合量和药物载量的增高,纤维材料复合程度加深。结论本研究提供了聚D,L-乳酸-co-乙醇酸电纺纤维中Poloxamer188及米托蒽醌载量对纤维形态及释药行为影响的初步信息。

Objective To prepare mitoxantrone-loaded poly D and L-lactic acid-co-glycolic acid electrospun fibers,and to investigate effects of Poloxamer 188 compound quantity and drug loading on fiber morphology and drug release.Methods Mitoxantrone,Poloxamer188,poly D and L-lactic acid-co-glycolic acid were all dissolved in dichloromethane and acetic acid cosolvent to be electrospun to form homogeneous solution.Scanning electron microscopy was used to observe the morphology of fibers.High performance liquid chromatograph-ultra violet spectrometry was used to determine the release amount of drug,and the release curves were drawn.Materials composite was characterized by X-ray diffraction and differential thermal analysis.Results Both the increased Poloxamer188 compound quantity and mitoxantrone loading amount in the system facilitated the formation of fibers with desirable morphology and drug release at the same time.Burst release amount of each fiber was all considerably big,with the sustained release period of less than 24 h.Release increment during 40-day platform period was very small.As Poloxamer188 composite volume and drug loading amount increased,composite degree of fiber materials was improved.Conclusion This study provided preliminary knowledge of how Poloxamer188 and mitoxantrone loading amount affected the morphology and drug release behavior of poly D and L-lactic acid-co-glycolic acid electrospun fiber.