烯效唑对葡萄副梢的影响及氮代谢关键基因表达分析

Effects of Uniconazole on Growth of Grapevine Accessory Shoots and Expression analysis of Key Genes for Nitrogen Metabolism

以‘着色香’和‘香悦’葡萄为试材,通过叶面喷施300 mg·L^(-1)烯效唑,开展烯效唑调控葡萄副梢生长发育的研究。结果表明,烯效唑处理可显著抑制‘着色香’葡萄副梢生长,副梢长度缩短55.97%,节间长度缩短22.32%,叶面积减少39.42%,相对叶绿素含量提高13.89%;而烯效唑调控‘香悦’葡萄副梢生长作用不显著。同时,通过5个葡萄氮代谢关键基因的半定量RT-PCR表达分析表明,‘着色香’副梢响应烯效唑调控的主效基因可能是亚硝酸还原酶基因(VvNiR),其表现为早期响应且表达贯穿整个调控时期。

In this experiment,the effects of uniconazole on the growth and development of grape accessory shoots were carried out by spraying 300 mg·L^(-1)of uniconazole on the leaves of two grape varieties(Zhuosexiang,Xiangyue).The results showed that the effect of uniconazole on the growth of the accessory shoots of the'Zhuosexiang'was significantly inhibited.After uniconazole treatment,the accessory shoot length,internode length,leaf area and relative chlorophyll content of'Zhuosexiang'were reduced by 55.97%,22.32%,39.42%and 13.89%respectively.However,the effect of uniconazole on regulating the growth of auxiliary shoots of'Xiangyue'was not significant.Meanwhile,the expression analysis of five key genes in grape nitrogen metabolism by semi-quantitative RT-PCR showed that VvNiR could be the main gene in response to uniconazole regulation in the growth of'Zhuosexiang'accessory shoots,it appeared as early response and expression throughout the regulatory period.