高表达lncRNA调控意大利蜜蜂工蜂中肠发育的作用解析

Analysis of highly-expressed lncRNAs function in regulating midgut development of Apis mellifera ligustica worker

为了探究高表达lncRNA(highly-expressed lncRNA,HlncRNA)调控意大利蜜蜂(Apis mellifera ligustica,简称意蜂)工蜂中肠发育的潜在作用,本研究利用前期获得的组学数据筛选出意蜂7和10日龄工蜂中肠的HlncRNA,通过RT-PCR验证上述HlncRNA的真实表达,通过RT-qPCR分析HlncRNA在1~21日龄工蜂中肠发育过程的表达谱,进而对HlncRNA的调控作用进行生物信息学分析.结果表明7和10日龄工蜂中肠中表达量最高的9条lncRNA相同;这些HlncRNA在1~21日龄工蜂中肠发育过程中真实表达且表达规律不同;HlncRNA通过顺式和反式作用分别调控32个上下游基因和18条共表达mRNA,此外可靶向55个miRNA进而结合131条mRNA.研究结果揭示上述9条HlncRNA可通过顺式作用、反式作用和ceRNA网络潜在调节应激反应与糖类消化和吸收等功能条目和通路,进而调控意蜂工蜂的中肠的发育.

In order to explore the potential role of highly-expressed lncRNAs(HlncRNAs)in regulating the midgut development of Apis mellifera ligustica worker,the omics data obtained in the previous at 7-and 10-day-old A.m.ligustica workers’midguts were used to screen out the HlncRNAs.RT-PCR was performed to confirm the real expression of aforementioned HlncRNAs and analysis the expression pattern of them during the developmental process of 1~21 days old workers’midguts,and bioinformatic analyses of the regulatory function of HlncRNAs were further performed.The results suggested that the 9 HlncRNAs in the 7 and 10-day-old worker’s midgut were the same.These HlncRNAs were expressed in 1-to 21-day-old workers’midguts but their expression patterns were different.HlncRNAs regulated 32 upstream and downstream genes and 18 co-expressed mRNAs via cis-acting and trans-acting manner,respectively.Additionally,these HlncRNAs can target 55 miRNAs and further bind to 131 mRNAs.These results reveal the 9 HlncRNAs potentially regulated functional terms and pathways such as the stress response,sugar digestion and absorption through cis-acting,trans-acting and ceRNA network to regulate the development of A.m.ligustica worker’s midgut.