Wnt2在植入胎盘组织中的表达及其对滋养细胞迁移和侵袭功能的影响

The expression of Wnt2 in placenta tissues of placenta accreta and its effect on the biological function of trophoblast cells

目的检测Wnt2在植入胎盘组织中的表达,分析Wnt2对滋养细胞凋亡、迁移和侵袭功能的影响。方法收集2020年7月至2021年1月于武汉大学中南医院妇产科行剖宫产的胎盘植入孕产妇胎盘组织15例(植入组)和正常孕产妇胎盘组织15例(对照组)。RT-PCR法和Western blot法检测胎盘组织中Wnt2表达水平,采用siRNA敲低HTR-8/Svneo细胞中Wnt2表达水平,流式细胞学法检测细胞凋亡,Transwell侵袭和迁移实验评估细胞的迁移和侵袭能力,RT-PCR法和Western blot法检测β-catenin、Bcl-2、Caspase-3、MMP-2和MMP-9表达水平。结果与对照组相比,植入组胎盘组织中Wnt2、MMP-2和MMP-9 mRNA和蛋白表达量均显著升高(均P<0.05)。转染敲低HTR-8/Svneo细胞Wnt2水平后,细胞凋亡率显著增加(P<0.05),细胞迁移和侵袭能力显著抑制(P<0.01),β-catenin、Bcl-2、MMP-2和MMP-9表达显著下降(均P<0.05),而Caspase-3表达显著升高(P<0.05)。结论植入胎盘组织中Wnt2蛋白表达显著升高,且Wnt2可能通过wnt/β-catenin信号通路参与了胎盘滋养细胞的凋亡、迁移和侵袭。

Objective:To detect and analyze the expression of Wnt2 in the placental accreta tissue of the placenta,to explore the effect of Wnt2 on the the apoptosis,migration and invasion functions of trophoblast cells.Methods:A total of fifteen cases(placenta accreta group)of the placental tissues of pregnant with placenta accreta who underwent cesarean section in our hospital from July 2020 to January 2021 were collected.Fifteen cases of normal placental tissues were selected during the same period as controls(control group).RT-PCR and Western blot were used to detect the expression level of Wnt2 in the placenta tissues of the two groups.After knocking down the expression level of Wnt2 in HTR-8/Svneo cells by siRNA,flow cytometry was used to detect cell apoptosis.Transwell invasion test and migration test were used to evaluate the migration and invasion ability of cells.RT-PCR and Western blot were used to detect the expression levels ofβ-catenin、Bcl-2、Caspase-3、MMP-2 and MMP-9.Results:Compared with the control group,the relative expressions of Wnt2,MMP-2 and MMP-9 mRNA and protein in the placenta tissue of the implantation group were significantly increased(all P<0.05).After the Wnt2 expression level of HTR-8/Svneo cells was knocked down,the apoptosis rate was significantly increased(P<0.01),and the migration and invasion ability of HTR-8/Svneo cells was inhibited(both P<0.01).The expressions ofβ-catenin、Bcl-2、MMP-2 and MMP-9 in the cells of the si-Wnt2 group decreased significantly(both P<0.05),while the expression of Caspase-3 increased significantly(P<0.05).Conclusion:The expression of Wnt2 protein in the placenta tissue of placenta accreta significantly increased,and Wnt2 may participate in the apoptosis,migration and invasion of placental trophoblast cells via wnt/β-catenin signaling pathway.