基于工业园农田土壤和植物的阴离子UE-SPE-IC方法研究

Study on Anion UE-SPE-IC Method for Soil and Plant in Industrial Park

为对污染土壤和植物样品进行快速检测,解决传统检测方法效率低下的问题,本研究提出UE-SPE-IC(超声-固相萃取-离子色谱)法测定农田土壤和植物中F^(-)、Cl^(-)、NO_(2)^(-)、NO_(3)^(-)、SO_(4)^(2-)和H_(2)PO_(4)^(-)6种阴离子的含量及污染程度。采用ICS-900型离子色谱仪及Dionex AS23型色谱柱(250mm×4mm)阴离子交换柱,以3mmol·L^(-1) NaHCO_(3)-2.4mmol·L^(-1) Na2CO_(3)溶液为淋洗液,流量为3mL·min^(-1)。本研究以某工业园附近受污染影响的农田土壤和植物为采集样品,实验结果显示,6种阴离子的浓度与各自对应的峰面积呈良好的线性关系,相关系数为0.9997~0.9999,检出限为0.005~0.023mg·L^(-1)(S/N=3)。样品的加标回收率为95.2%~104.82%,测定结果的相对标准偏差为0.27%~9.3%。实验结果证明UE-SPE-IC法具有操作简单、准确度高、检测时间短、安全性高等优点,能够满足土壤样品或植株样品中F^(-)、Cl^(-)、NO_(2)^(-)、NO_(3)^(-)、SO_(4)^(2-)和H_(2)PO_(4)^(-)6种阴离子的测定要求。

In order to conduct rapid detection of contaminated soil and plant samples and solve the problem of low efficiency of traditional detection methods,a method for the determination of F^(-),Cl^(-),NO_(2)^(-),NO_(3)^(-),SO_(4)^(2-) and H_(2)PO_(4)^(-)6anions in farmland soil and plant by UE-SPE-IC(ultrasonic-solid phase extraction-ion chromatography)is proposed in this paper.ICS-900ion chromatograph and Dionex AS23column(250mm×4mm)anion exchange column were used.3mmol·L^(-1) NaHCO_(3)-2.4mmol·L^(-1) Na2CO_(3)solution was used as eluent and the flow rate was 3mL·min^(-1).In this paper,the soil and plants of the polluted farmland near an industrial park were taken as samples,the experimental results show that there is a good linear relationship between the concentrations of the six anions and their corresponding peak areas,the correlation coefficient is 0.9997-0.9999,and the detection limit is 0.005—0.023mg·L^(-1)(S/N=3).The recovery rate of the sample is 95.2%—104.82%,and the relative standard deviation of the determination result is 0.27%—9.3%.The experimental results show that UE-SPE-IC method has the advantages of simple operation,high accuracy,short detection time,high integrity,and can meet the requirements of soil samples or plant samples for the determination of F^(-)、Cl^(-)、NO_(2)^(-)、NO_(3)^(-)、SO_(4)^(2-) and H_(2)PO_(4)^(-)6anions.