凹纹胡蜂蜂毒中一种新型多肽的识别与活性检测

Identification and Viability Assay of a New Peptide in Venom of Vespa velutina

【目的】从凹纹胡峰蜂毒中识别出具有生物活性的新多肽,为后续相关药物的开发奠定基础。【方法】提取胡蜂毒囊中的RNA,构建cDNA文库,利用特殊引物筛选出目标基因,以此识别新多肽,并对新多肽的生物活性进行检测。【结果】得到新的凹纹胡蜂蜂毒多肽,并命名为VV-VP-2;其质量浓度为100~200μg/mL时对金黄色葡萄球菌(Staphylococcus aureus)有抑制作用,质量浓度为50~200μg/mL时对肺炎克雷伯菌(Klebsiella pneumoniae)有抑制作用。此外,5~200μg/mL的VV-VP-2对DPPH和ABTS自由基均有极显著的清除能力(P<0.01或P<0.001),50~200μg/mL的VV-VP-2对·OH自由基具有极显著的清除能力(P<0.01或P<0.001)。【结论】新多肽VV-VP-2具有开发成为抗菌和抗氧化药物的潜力,可为综合性药物或保健品的开发奠定基础。

[Purpose]To identify new peptides with biological activity from the venom of Vespa velutina,and lay a foundation for the subsequent development of related drugs.[Methods]RNA was extracted from the venoms of V.velutina,cDNA library was constructed,and target genes were screened by special primers to identify new peptides and detect their biological activity.[Results]The new peptide was named VV-VP-2.Peptide VV-VP-2 could inhibit Staphylococcus aureus at 100-200μg/mL and Klebsiella pneumoniae at 50-200μg/mL.In addition,5-200μg/mL VV-VP-2 had an extremely significant scavenging ability on DPPH and ABTS radical(P<0.01or P<0.001),and 50-200μg/mL VV-VP-2 had an extremely significant scavenging ability on·OH radical(P<0.01 or P<0.001).[Conclusion]The new peptide found in this study have the potential to be developed as antibacterial and antioxidant drugs,which can lay a foundation for the development of comprehensive drugs or health care products.