IL-17A基因多态性与过敏性紫癜易感性及紫癜性肾炎的相关性研究

Association between the polymorphisms of the interleukin 17A gene promoters and susceptibility to henoch-sehtlnlein purpura

目的:探讨白细胞介素-17A(IL-17A)的-197G/A和-692C/T两个位点基因多态性与儿童过敏性紫癜(HSP)易感性及紫癜性肾炎(HSPN)的关系。方法:选取380例川南地区汉族HSP患儿(HSP组)及同地区同时间段405名健康儿童(对照组)为研究对象,患病组依据有无肾脏损伤分为紫癜性肾炎组(HSPN组,n=115)和非紫癜性肾炎组(非HSPN组,n=265)。比较各组儿童IL-17A基因-197G/A和-692C/T两个位点单核苷酸多态性(SNP)的分布,分析其与HSP易感性及HSPN的关系。结果:IL-17A-692C/T位点基因型TT在HSP组分布高于对照组(P<0.001),携带T等位基因的儿童罹患HSP的风险是携带C等位基因的1.390倍(95%CI:1.139~1.697,P=0.001);而基因型及等位基因的分布频率在-197G/A位点在HSP组和对照组间的差异无统计学意义(P>0.05)。HSPN组与非HSPN组IL-17A基因两个位点的基因型和等位基因分布比较,差异无统计学意义(P>0.05)。结论:川南地区汉族儿童中HSP的易感性与IL-17A-692C/T基因多态性相关,携带-692位点T等位基因的儿童更易罹患HSP,而IL-17A-197G/A位点多态性与该地区儿童HSP的易感性无相关性,IL-17A两个基因的多态性与儿童HSP肾脏损害亦无相关性。

Objective:To investigate the relationship between-197 G/A and-692 C/T gene polymorphisms of interleukin-17 A(IL-17 A) and susceptibility to Henoch Schonlein purpura(HSP) and Henoch Schonlein nephritis(HSPN) in children.Methods:380 children with HSP of Han nationality in Southern Sichuan(HSP group) and 405 healthy children(control group) at the same time in the same area were selected as the research objects.The sick group was divided into purpura nephritis group(HSPN group,n=115) and non purpura nephritis group(non HSPN group,n=265).The distribution of single nucleotide polymorphisms(SNPs) at-197 G/A and-692 C/T of IL-17 A gene in children of each group was compared,and its relationship with HSP susceptibility and HSPN was analyzed.Results:The frequency distribution of TT at-692 C/T loci of IL-17 A gene promoter in the HSP group was significantly higher than that in the control group(P<0.001).In addition,the amount of carrier of the T allele was 1.390 times,which was higher than the carrier of the C allele in children with HSP(95% CI:1.139-1.697,P=0.001).Moreover,the frequency distribution of-197 G/A in the IL-17 A gene was not significantly different between the HSP group and the control group(P>0.05).Furthermore,the frequency distribution of the IL-17 A gene(-692 C/T and-197 G/A) had no significant correlation between the HSP nephritis(HSPN) group and the non-HSPN group(P>0.05).Conclusion:IL-17 A gene promoter polymorphism at position-692 is related to HSP susceptibility in South Sichuan of China.-692 T allele carriers are more susceptible to HSP,no significant correlation between SNP at-197 G/A in IL-17 A promoter and susceptibility to childhood HSP is observed in this area,and no correlation between IL-17 A gene polymorphism and HSP renal damage in children.