消积抑癌外敷方对Lewis肺癌荷瘤小鼠抑瘤效果和肿瘤血管生成的影响

Influence of Xiaoji Yi’ai external application recipe on anti-tumor effect and tumor angiogenesis in tumor-bearing Lewis lung cancer mice

目的:探讨消积抑癌外敷方对Lewis肺癌荷瘤小鼠抑瘤效果、肿瘤血管生成、血管内皮生长因子(VEGF)蛋白及mRNA表达的影响。方法:将20只健康雌性C57BL/6小鼠随机分为荷瘤对照组和治疗组,每组各10只。采用动物移植肿瘤实验法制备Lewis肺癌荷瘤小鼠模型。对照组小鼠瘤体表面外敷面糊,治疗组小鼠瘤体表面外敷消积抑癌膏,疗程均为30 d。疗程结束后剥离肿瘤组织称重瘤质量,CD34染色标记微血管计数微血管密度,免疫组化法检测VEGF蛋白表达,Western blot检测VEGF蛋白表达量,RT-qPCR检测VEGF mRNA表达量。结果:治疗组瘤质量少于对照组(P<0.05),且微血管密度低于对照组(P<0.05)。对照组和治疗组的VEGF表达均为阳性,且治疗组VEGF表达的阳性等级低于对照组(P<0.05)。治疗组VEGF蛋白和mRNA表达量均低于对照组(P<0.05)。结论:消积抑癌外敷方可下调Lewis肺癌荷瘤小鼠VEGF表达,抑制肿瘤血管生成,减少瘤质量,有一定的抗肿瘤作用。

Objective:To explore the influence of Xiaoji Yi’ai external application recipe on anti-tumor effect,tumor angiogenesis,protein and mRNA expression of vascular endothelial growth factor(VEGF) in tumor-bearing Lewis lung cancer mice.Methods:20 healthy female C57 BL/6 mice were randomly divided into tumor-bearing control group and treatment group,with 10 mice in each group.The tumor-bearing Lewis lung cancer mouse model was prepared by animal tumor transplantation experiment method.The tumor-bearing control group was given external application of batter on tumor surface,the treatment group was given external application of Xiaoji Yi’ai ointment on tumor surface,the course of treatment was 30 days.After treatment,tumor tissues were stripped,the mass of tumor was measured,paraffin sections were prepared,microvessels were marked by CD34 staining,and microvessel density was calculated.Tumor tissue homogenate was collected to detect the expression level of VEGF protein by Western blot,and VEGF mRNA was detected by RT-qPCR.Results:The mass of tumor and microvessel density in the treatment group were lower than those in the tumor-bearing control group(P<0.05).The expression of VEGF was positive in tumor-bearing control group and treatment group.The positive expression grade of VEGF in treatment group was lower than that in tumor-bearing control group(P<0.05).The expression level of VEGF protein and VEGF mRNA in treatment group were lower than those in tumor-bearing control group(P<0.05).Conclusion:Xiaoji Yi’ai external application recipe can down-regulate the expression of VEGF in tumor-bearing Lewis lung cancer mice,inhibit tumor angiogenesis,reduce tumor quality,and have a certain anti-tumor effect.