基于SLAF_seq的重组自交系群体定位水稻株高QTL

Mapping of QTLs for Plant Height Using SLAF_seq Recombinant Inbred Lines Population in Rice

株高是水稻最重要的农艺性状之一,定位和克隆水稻株高基因具有重要的现实意义。本研究利用矮秆品种‘V20B’和高秆品种‘CPSLO17’作为亲本,构建了150个重组自交系家系(RILs),结合SLAF_eq技术构建的水稻高密度遗传图谱,对水稻株高进行QTL检测。利用MapQTL5进行区间作图,阈值设定为2.5,在第1和第6染色体各检测到2个株高QTL位点,分别命名为qPH1-1、qPH1-2、qPH6-1和qPH6-2,这4个QTL位点的LOD值分别为3.2、10.4、3.1和5.6,对表型变异的贡献率分别为6.5%、22.2%、6.0%和11.6%。其中,qPH1-1、qPH1-2和qPH6-2增效位点来自于‘CPSLO17’,而qPH6-1增效位点来自于‘V20B’。本研究为进一步克隆水稻株高QTLs,进而阐明株高的分子调控机制提供参考依据。

Plant height is one of the most important agronomic traits of rice,and mapping and cloning the genes controlling the plant height of rice have vital significance.This study used 150 recombinant inbred line(RILs),derived from a cross between’V20 B’and’CPSLO17’.Using a rice high-density genetic linkage map of SLAF makers and interval mapping,performed by MapQTL5,to detect rice plant height QTLs.Two QTLs for plant height were detected on chromosome 1 and 6 respectively,named qPH1-1,qPH1-2,qPH6-1 and qPH6-2,individual QTL(LOD=3.2,LOD=10.4,LOD=3.1 and LOD=5.6)explained 6.5%,22.2%,6.0%and 11.6%of the observed phenotype variance,and the effect-increasing of qPH1-1,qPH1-2 and qPH6-2 loci were originated from’CPSLO17’,while the effect-increasing of qPH6-1 loci was originated from’V20 B’.Therefore,this study provides a reference for further cloning of plant height QTLs,and then to clarify the molecular regulation mechanism of plant height in rice.