多房棘球蚴感染小鼠脾脏巨噬细胞亚群及其极化表型的变化

Changes of macrophage subsets and polarization in spleen of mice infected with Echinococcus multilocularis

目的研究不同数量多房棘球蚴原头节感染小鼠脾巨噬细胞亚群及其极化表型的变化。方法60只C57BL/6小鼠随机分为低、中、高感染组和对照组,每组15只。低、中、高感染组每只小鼠分别经肝门静脉穿刺接种50、500和2000个多房棘球蚴原头节,对照组注射等量的生理盐水。感染后2、12和24周,各组随机选5只小鼠摘取脾脏,称量后计算脾脏系数;分离脾淋巴细胞,采用流式细胞术检测各组小鼠脾巨噬细胞不同亚群比例和数量;提取小鼠脾淋巴细胞总RNA,实时荧光定量PCR检测巨噬细胞M1和M2极化相关基因的m RNA相对转录水平,其中M1型极化相关基因为白细胞介素-1β(IL-1β)、γ干扰素(IFN-γ)、趋化因子(C-X-C基序)配体11(CXCL11)、趋化因子(C-C基序)受体7(CCR7)和CD86,M2型极化相关基因为甘露糖受体C-1型(MRC1)、抵抗素样分子α(Retnla)和精氨酸酶1(ARG1),以甘油醛-3-磷酸脱氢酶(GAPDH)为内参。采用SPSS 26.0软件进行统计学分析,不同时间点多组间的比较采用单因素方差分析,同一感染时间点的两两比较采用LSD法检验。结果感染后2周,高感染组小鼠脾脏质量、脾脏系数、脾淋巴细胞数量、脾巨噬细胞比例、脾巨噬细胞数量和Ly-6C高表达(Ly-6C^(high))巨噬细胞的比例分别为(157.2±22.8)mg、(0.8±0.1)%、(10.3±2.9)×10^(7)、(9.2±6.4)%、(48.9±32.7)×10^(5)和(75.8±4.6)%,均高于对照组[(75.0±18.3)mg、(0.4±0.1)%、(3.1±1.3)×10^(7)、(4.3±0.7)%、(7.7±4.1)×10^(5)、(52.1±8.4)%]、低感染组[(89.2±7.4)mg、(0.4±0.0)%、(5.4±2.3)×10^(7)、(3.0±0.9)%、(9.3±6.9)×10^(5)、(50.1±8.8)%]和中感染组[(102.6±15.2)mg、(0.5±0.1)%、(7.0±2.1)×10^(7)、(3.5±0.3)%、(13.7±3.9)×10^(5)、(60.3±8.7)%](F=22.744、23.542、9.318、3.935、6.617、11.197,P<0.05或P<0.01);Ly-6C低表达(Ly-6Clow)巨噬细胞比例(20.3±4.2)%低于对照组(39.0±7.1)%、低感染组(41.2±8.6)%和中感染组(34.2±7.1)%(F=9.157,P<0.01)。感染后12周,高感染组小鼠脾脏质量、脾脏系数、脾巨噬细胞比例、巨噬细胞数量以及Ly-6C^(high)巨噬细胞比例均高于对照组、低感染组和中感染组(F=12.730、14.173、20.380、7.943和25.838,P<0.01);Ly-6Clow巨噬细胞比例低于对照组、低感染组和中感染组(F=27.668,P<0.01)。感染后24周,高感染组小鼠脾脏质量、脾脏系数、脾淋巴细胞数量、脾巨噬细胞比例和数量均高于对照组、低感染组和中感染组(F=8.664、7.318、13.047、3.315、6.007,P<0.05或P<0.01);Ly-6C^(high)和Ly-6Clow巨噬细胞比例与对照组、低感染组和中感染组差异无统计学意义(F=3.177、2.709,P>0.05),且随着感染时间的延长,Ly-6C^(high)巨噬细胞比例逐渐降低(F=30.649,P<0.01),Ly-6Clow巨噬细胞比例逐渐升高(F=32.407,P<0.01)。实时荧光定量PCR检测结果显示,感染后24周,高感染组M1型极化基因CXCL11的mRNA相对转录水平(28.2±36.3)高于对照组(1.9±2.7)(t=2.243,P<0.05),CD86(0.2±0.1)低于对照组(0.5±0.3)(t=-2.255,P<0.05);M2型极化基因MRC1、Retnla和ARG1的mRNA相对转录水平(5.2±2.9、201.8±176.4、51.2±69.6)均高于对照组(1.8±1.5、0.8±0.8、1.2±0.8)(t=2.313、3.470、2.185,P<0.05)。结论感染后2周,多房棘球蚴原头节高感染组小鼠脾脏募集大量Ly-6C阳性单核来源的巨噬细胞,感染进程中Ly-6C^(high)巨噬细胞逐渐向Ly-6Clow转变,且在感染后12周和24周脾巨噬细胞以M2型为主,诱导小鼠免疫耐受,利于多房棘球蚴的慢性寄生。

Objective To investigate the changes of splenic macrophages subsets and their polarization phenotype in mice infected with different numbers of Echinococcus multilocularis protoscoleces.Methods Sixty female C57BL/6 mice were randomly assigned to mild,moderate,and heavy infection groups and control group with15 mice in each group.The mice in mild,moderate and heavy infection groups were inoculated with 50,500,and2000 protoscoleces via hepatic portal vein puncture,respectively,while the control group was injected with the same volume of saline.At 2,12,and 24 weeks after infection,the spleens from five mice randomly selected from each group were collected,followed by calculating the spleen coefficients after weighted,and isolating splenic lymphocytes.The proportions and numbers of different macrophages subsets were determined by flow cytometry.The total RNA of splenic lymphocytes was extracted,and the relative level of mRNA transcription of M1 and M2 polarization related genes in splenic macrophages were examined with real-time quantitative PCR.The M1 type polarization related genes were those involving interleukin-1β(IL-1β),interferon-γ(IFN-γ),chemokine(C-X-C motif)ligand 11(CXCL11),chemokine(C-C motif)receptor 7(CCR7)and CD86,and the M2 type polarization related genes were involving mannose receptor C-type 1(MRC1),resistin like alpha(Retnla)and arginase 1(ARG1).Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)was used as an internal reference.SPSS 26.0 software was used for statistical analysis.The comparison between multiple groups at different time points was analyzed by one-way ANOVA,and the pairwise comparative analysis at the same time point was tested by LSD method.Results Two weeks after infection,the spleen weight,spleen coefficient,the number of splenic lymphocytes,the proportion of splenic macrophages,the number of splenic macrophages and Ly-6C^(high)splenic macrophage subset proportion in heavy infection group were(157.2±22.8)mg,(0.8±0.1)%,(10.3±2.9)×10^(7),(9.2±6.4)%,(48.9±32.7)×10^(5)and(75.8±4.6)%,respectively,which were all significantly higher than that in the control group[(75.0±18.3)mg,(0.4±0.1)%,(3.1±1.3)×10^(7),(4.3±0.7)%,(7.7±4.1)×10^(5),(52.1±8.4)%],mild infection group[(89.2±7.4)mg,(0.4±0.0)%,(5.4±2.3)×10^(7),(3.0±0.9)%,(9.3±6.9)×10^(5),(50.1±8.8)%],and in moderate infection group[(102.6±15.2)mg,(0.5±0.1)%,(7.0±2.1)×10^(7),(3.5±0.3)%,(13.7±3.9)×10^(5),(60.3±8.7)%](F=22.744,23.542,9.318,3.935,6.617,11.197,P<0.05 or P<0.01).Ly-6Clowmacrophage subset proportion(20.3±4.2)%was significantly milder than that in the control group(39.0±7.1)%,mild infection group(41.2±8.6)%and in moderate infection group(34.2±7.1)%(F=9.157,P<0.01).At 12 weeks after infection,the spleen weight,spleen coefficient,the proportion and number of macrophages and the proportion of the Ly-6C^(high)macrophage subset in the spleen were significantly higher in heavy infection group than that in the control group,mild and moderate infection groups(F=12.730,14.173,20.380,7.943,25.838,P<0.01),the proportion of Ly-6Clowmacrophages was significantly lower than that in the control group,mild and moderate infection groups(F=27.668,P<0.01).At 24weeks after infection,the spleen weight,spleen coefficient,the number of lymphocytes,the proportion and number of macrophages in the spleen were also significantly higher in heavy infection group(F=8.664,7.318,13.047,3.315,6.007,P<0.05 or P<0.01).The proportions of Ly-6C^(high)and Ly-6Clowmacrophages were not significantly different from those of the control group,mild and moderate infection groups(F=3.177 and 2.709,P>0.05).With longer time of infection,the proportion of Ly-6C^(high)macrophages was gradually decreased(F=30.649,P<0.01),and the proportion of Ly-6Clowmacrophages was gradually increased(F=32.407,P<0.01).Real-time quantitative PCR showed that at 24 weeks after infection,the relative level of mRNA transcription of CXCL11 in heavy infection group(28.2±36.3)was higher than that in control group(1.9±2.7)(t=2.243,P<0.05),and the relative level of m RNA transcription of CD86(0.2±0.1)was significantly lower than that in the control group(0.5±0.3)(t=-2.255,P<0.05).The relative transcription level of mRNA of MRC1,Retnla,and ARG1(5.2±2.9,201.8±176.4,51.2±69.6)were significantly higher than those in control group(1.8±1.5,0.8±0.8,1.2±0.8,respectively)(t=2.313,3.470 and 2.185,P<0.05)Conclusion Two weeks after infection,large numbers of Ly-6C positive monocyte derived macrophages were recruited into spleen in the heavy infection group and the Ly-6C^(high)macrophage subset gradually transformed shifted to the Ly-6Clowsubset during infection.At 12 weeks and 24 weeks after infection,the macrophages in the spleen were dominated by the M2 type,inducing immune tolerance,favorable to chronic infection of Echinococcus multilocularis metacestode.