番茄ERFb.2转录因子的克隆、生物信息学及表达特征分析

Cloning, Bioinformatics and Expression Analysis of ERFb.2 Transcription Factor in Tomato

通过克隆番茄SlERFb.2基因,并运用生物信息学对该基因的编码产物进行一级、二级、三级结构分析,并通过qRT-PCR技术检测SlERFb.2基因在不同处理不同时期的表达量,以期为番茄对非生物胁迫的响应机制提供参考依据。结果表明:SlERFb.2基因含有开放阅读框5个,共编码258个氨基酸,属于酸性蛋白;其二级结构以α-螺旋为主,预测蛋白定位于细胞核上;且含有39个磷酸化位点与15个糖基化位点;启动子区主要以I-box为主,且含有多个诱导基因表达的顺势作用元件;三级结构相对简单,且该转录因子在番茄中存在4个互作蛋白,系统发育树得番茄与马铃薯的亲缘关系最近;qRT-PCR显示该基因对低温的响应特别敏感。

The SlERFb.2 gene of tomato was cloned, and the structure of the encoded protein was analyzed by using bioinformatics methods, and the expression level of the SlERFb.2 was detected by qRT-PCR in different treatments and different periods, in order to provide some theoretical basis for the response mechanism of tomato to abiotic stress.The results showed that the SlERFb.2 contained 5 open reading frames, encoding a total of 258 amino acids, which was an acidic protein;its secondary structure was mainly α-helix, and the predicted protein was located on the nucleus;and contained 39 phosphorylation sites with 15 glycosylation sites;the promoter region was mainly I-box, and contained multiple homeopathic elements that induce gene expression;the tertiary structure was relatively simple, and the transcription factor had 4 interactions in tomato protein, phylogenetic tree, the closest relationship between tomato and potato;qRT-PCR showed that the gene was particularly sensitive to low temperature response.