Rab27a与E-cadherin在复发性流产中的表达及关系研究

Study on the expression and relationship of Rab27a and E-cadherin in patients with recurrent abortion

目的通过检测复发性流产患者蜕膜组织中Rab27a和E-cadherin的表达水平及对二者做相关性分析,探讨Rab27a和E-cadherin在复发性流产中的临床意义。方法选取2017年12月至2019年12月于河北医科大学第四医院妇产科因复发性流产行清宫术的患者66例为实验组,同期因意外妊娠要求终止的50例为对照组,术后取蜕膜组织作为检测标本。利用免疫组化(immunohistochemistry)方法分别检测蜕膜组织中Rab27a和E-cadherin表达水平,并对两者表达水平做相关性分析。结果(1)复发性流产与年龄、体重指数、痛经、妊娠时间及辅助生殖相关,差异有统计学意义(P<0.05);与血红蛋白数、白细胞数、孕次、产次不相关,差异无统计学意义(P>0.05);(2)Rab27a在实验组较对照组表达增高,两组比较差异有统计学意义(P<0.05);(3)E-cadherin在实验组较对照组表达降低,两组比较差异有统计学意义(P<0.05);(4)Rab27a与E-cadherin在复发性流产蜕膜组织中表达水平呈负相关。结论Rab27a在复发性流产蜕膜组织中呈现高表达,E-cadherin呈现低表达,两者表达呈负相关。Rab27a可能通过降低E-cadherin的表达从而参与复发性流产的致病过程。

Objective To explore expression pattern of Rab27 a and E-cadherin and their clinical relevance in recurrent pregnancy loss.Methods 66 pregnant women who experienced recurrent pregnancy loss from December 2017 to December 2019 were collected as the study group in the Fourth Hospital of Hebei Medical University,while 50 pregnant women who underwent normal abortion were taken as the control group.Expression of Rab27 a and E-cadherin protein in decidual tissues were measured by immunohistochemistry.Correlation analysis was performed to survey the association of Rab27 a and E-cadherin expression in recurrent pregnancy loss.Results(1)Recurrent pregnancy loss were correlated with age,body mass index,dysmenorrhea,pregnancy time and IVF-ET.No significant difference was observed in hemoglobin number,leukocyte number,pregnancy times,and parity between the two groups.(2)The expression of Rab27 a of the study group was significantly higher than the control group(P<0.05).(3)The expression of E-cadherin of the study group was significantly lower than control group(P<0.05).(4)The Rab27 a and E-cadherin expression were demonstrated negative correlation in recurrent pregnancy loss(r=-0.301,P=0.014).Conclusions Expression of Rab27 a and E-cadherin are negatively correlated in recurrent pregnancy loss.High Rab27 a and low E-cadherin might be biomarker of recurrent pregnancy loss.