麸炒苍术粗多糖对脾虚模型大鼠的影响

Effect of polysaccharide of bran-fried Atractylodis Rhizoma on rats of spleen deficiency model

目的探究麸炒苍术粗多糖对脾虚模型大鼠的影响。方法SD雄性大鼠随机分为空白组、模型组、麸炒苍术组、多糖低、中、高剂量组,每组10只。采用饮食不节、疲劳过度加苦寒泻下的复合因素法复制脾虚模型,灌胃给药7 d。考察造模后大鼠体质量、粪便含水量和免疫器官指数,酶联免疫吸附法(ELISA)测定生长抑素(SS)、血管活性肠肽(VIP)、胃泌素(GAS)、淀粉酶(AMS)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、免疫球蛋白(IgG)、白介素4(IL-4)、球蛋白(GLB)和总蛋白(TP)水平。结果与模型组相比,多糖各剂量组均能显著升高脾虚大鼠体质量(P<0.01),降低粪便含水率(P<0.01);中剂量组和高剂量组能升高脾虚大鼠胸腺指数和脾脏指数(P<0.01);多糖各剂量组均能明显升高GAS、AMS和IL-4水平,降低SS、VIP、IL-6、TNF-α、IgG、GLB和TP水平(P<0.01)。结论麸炒苍术粗多糖对脾虚模型大鼠具有较好的治疗作用,可以初步确定多糖是苍术健脾的有效成分之一。

Objective To explore the effect of polysaccharides of bran-fried Atractylodis Rhizoma on rats of spleen deficiency model.Methods SD male rats were randomly divided into blank group,model group,bran-fried Atractylodis Rhizoma group,polysaccharide low,medium and high dose groups,with 10 rats in each group.A rat spleen-deficiency syndrome model was established by the compound factor method of dietary disorders,excessive fatigue associated with diarrhea induced by bitter cold herbs.The rats were administered by gavage for 7 days to investigate the body weight,fecal water content and immune organ index.Somatostatin(SS),vasoactive intestinal peptide(VIP),gastrin(GAS),amylase(AMS),interleukin 6(IL-6),tumor necrosis factorα(TNF-α),immunoglobulin(IgG),interleukin 4(IL-4),globulin(GLB)and total protein(TP)were determined by enzyme linked immunosorbent assay(ELISA).Results Compared with the model group,polysaccharides could significantly increase the body weight and decrease the water content of feces in spleen deficiency rats(P<0.01),increase thymus index and spleen index in medium and high dose groups(P<0.01),increase GAS,AMS and IL-4,and decrease SS,VIP,IL-6,TNF-α,IgG,GLB and TP in all dose groups polysaccharides(P<0.01).Conclusion Polysaccharide of bran-fried Atractylodis Rhizoma has a good therapeutic effect on rats with spleen deficiency.It can be preliminarily determined that the polysaccharide is one of the effective ingredients of Atractylodis Rhizoma for strengthening the spleen.