过表达FOXB2对肝癌细胞的增殖、迁移和侵袭的影响

Effect of overexpression of forkhead box B2 on proliferation,invasion and migration of hepatocellular carcinoma cells

目的探讨叉头框蛋白B2(FOXB2)对肝癌细胞增殖、迁移和侵袭能力的影响。方法采用定量PCR检测正常肝细胞LO2细胞和肝癌细胞系Hep3B、Huh7细胞FOXB2 mRNA的表达,构建过表达FOXB2的细胞模型,逆转录实时荧光定量PCR和蛋白免疫印迹法检测过表达效率,细胞计数试剂盒-8(CCK-8)法检测FOXB2对Huh7细胞增殖能力的影响;平板克隆实验检测FOXB2对Huh7细胞克隆形成能力的影响;Transwell实验检测FOXB2对Huh7细胞迁移和侵袭能力的影响。结果FOXB2在肝癌细胞系Huh7和Hep3B细胞中的表达水平低于正常肝细胞LO2(P<0.001);过表达FOXB2抑制了Huh7细胞的增殖(P=0.005)和克隆形成(P<0.001);FOXB2过表达肝癌细胞的迁移(P<0.001)和侵袭(P=0.002)能力低于Vector对照细胞。结论FOXB2在肝癌细胞中低表达,FOXB2过表达抑制了肝癌细胞的增殖、迁移和侵袭。

Objective To evaluate the effect of forkhead box protein B2(FOXB2)on the proliferation,invasion and migration of hepatocellular carcinoma cells.Methods The expression levels of FOXB2 mRNA in normal liver cells LO2,hepatocellular carcinoma cells Hep3B and Huh7 were detected by quantitative PCR.The cell model overexpressing FOXB2 was constructed.The overexpression efficiency was assessed by real-time fluorescent quantitative PCR and Western blot.The effect of FOXB2 on Huh7 cell proliferation was evaluated by cell counting kit-8(CCK-8).The effect of FOXB2 on the clone formation of Huh7 cells was determined by plate cloning assay.The effect of FOXB2 on the invasion and migration of Huh7 cells was detected by Transwell chamber test.Results The expression levels of FOXB2 in hepatocellular carcinoma cells Huh7 and Hep3B were significantly lower than that in normal liver cells LO2(both P<0.001).Overexpression of FOXB2 significantly inhibited the proliferation(P=0.005)and clone formation(P<0.001)of Huh7 cells.In the Huh7-FOXB2 overexpression group,the invasion(P=0.002)and migration capabilities(P<0.001)of hepatocellular carcinoma cells were significantly lower than that in the Vector control cells.Conclusions FOXB2 is lowly expressed in hepatocellular carcinoma cells.Overexpression of FOXB2 suppresses the proliferation,invasion and migration of hepatocellular carcinoma cells.