茶树TPP基因家族的鉴定及其对低温胁迫的响应

Identification and response of TPP gene family to low temperature stress in tea plant

[目的]本文旨在鉴定茶树海藻糖-6-磷酸磷酸酶(TPP)基因家族,并探索其在茶树抵御低温胁迫调控中的功能。[方法]利用生物信息学的方法,对茶树TPP基因家族进行进化树、保守结构域、启动子顺式元件等特性进行分析;通过茶树TPP基因家族的组织特异性表达及其对低温胁迫的响应,初步探讨CsTPP的生物学功能。[结果]从茶树基因组中鉴定到12条具有Trehalose-PPase保守结构域的CsTPP序列。系统进化树分析表明,根据与拟南芥、水稻的TPP基因同源性差异比较,可以将CsTPP分成3组。启动子顺式元件分析表明,CsTPP的启动子区含有多个胁迫响应相关元件和激素响应相关元件。基因表达分析显示,12个CsTPP基因在不同组织的表达量差异较大,具有明显的组织表达特异性。低温胁迫响应分析显示,4个CsTPP基因可能参与茶树叶片响应低温胁迫过程。[结论]CsTPP基因家族可能参与茶树响应低温胁迫的过程。

[Objectives]This article aimed to identify the trehalose-6-phosphate phosphatase(TPP)family genes of tea plant(Camellia sinensis),and explore their functions in the regulation of tea plants against low temperature stress.[Methods]The evolutionary relationship of the genes,the conserved domains of the coding protein,and the cis-elements of the promoter were analyzed by bioinformatics methods.Then,the expression of CsTPP in different tissues,and the response to low temperature stress were explored.[Results]Twelve CsTPP sequences with Trehalose-PPase conserved domains were identified from the tea plant genome.The phylogenetic analysis showed that CsTPP was divided into three groups according to their homology differences with TPP genes of Arabidopsis and Oryza sativa.The promoter analysis showed that CsTPP contained multiple stress response related and hormone response elements.Gene expression analysis showed that the expression levels of 12 CsTPP genes varied greatly in different tissues and had marked with tissue expression specificity.The analysis of response to cold stress showed that four CsTPP genes might be involved in tea leaves in response to cold stress process.[Conclusions]The CsTPP gene family might participate in low temperature stress of tea plant.