肺腺癌组织中LncRNA-p21的表达及其与病理参数、预后的关系

Expression of lncRNA-p21 in lung adenocarcinoma tissue and its relationship with pathological parameters and prognosis

目的探讨肺腺癌组织中长链非编码RNA(LncRNA)-p21表达及与临床病理参数和预后的关系。方法选择2015年1月至2017年12月徐州医科大学附属医院收治的96例肺腺癌患者,取手术切除的癌组织以及癌旁组织(距离癌组织边缘5 cm以上)采用实时荧光定量聚合酶链反应(qRT-PCR)检测组织中LncRNA-p21表达。分析LncRNA-p21表达与临床病理参数之间的关系。术后随访3年,采用Kaplan-Meier绘制不同LncRNA-p21表达肺腺癌患者生存曲线,Cox比例风险模型分析影响肺腺癌患者预后的因素。结果肺腺癌组织LncRNA-p21相对表达量低于癌旁组织(1.03±0.27 vs 3.02±0.81,P<0.05),其中,肿瘤直径≥3 cm、低中度分化、T_(3)期、N_(2)期患者LncRNA-p21相对表达量低于肿瘤直径<3 cm、高度分化、T_(1~2)期、N_(0~1)期患者(P均<0.05)。LncRNA-p21低表达患者的3年总生存率(OS)、无进展生存率(PFS)低于LncRNA-p21高表达患者(38.78%vs 63.83%,8.16%vs 34.04%,P均<0.05)。Cox比例风险回归分析显示,N分期(HR=1.881,95%CI:1.340~2.641,P<0.001)、LncRNA-p21低表达(HR=0.609,95%CI:0.453~0.819,P=0.001)是肺腺癌患者不良预后的影响因素。结论肺腺癌组织中LncRNA-p21表达下调,LncRNA-p21低表达与肺腺癌肿瘤大小、分化程度、T、N分期以及不良预后有关。

Objective To investigate the relationship between long non-coding ribonucleic acid(lncRNA)-p21 expression and clinicopathological parameters and prognosis in lung adenocarcinoma.Methods A total of 96 patients with lung adenocarcinoma admitted to the Affiliated Hospital of Xuzhou Medical University from January 2015 to December 2017 were selected,and the surgically resected cancer tissues and paracancerous tissues(more than 5 cm away from the edge of cancer tissues)were taken to detect the expression of lncRNA-p21 by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The relationship between lncRNA-p21 expression and clinicopathological parameters was analyzed.After 3 years of postoperative follow-up,Kaplan-Meier survival curves were plotted for lung adenocarcinoma patients with different lncRNA-p21 expression,and the prognostic factors of lung adenocarcinoma patients were analyzed with the Cox proportional risk model.Results The relative expression of lncRNA-p21 in lung adenocarcinoma tissues was lower than that in adjacent tissues(1.03±0.27 vs 3.02±0.81,P<0.05).The relative expression of lncRNA-p21 in patients with tumor diameter≥3 cm,low to moderate tumor differentiation,T_(3) stage,and N_(2) stage were lower than those in patients with tumor diameter<3 cm,high tumor differentiation,T_(1-2)stage,and N_(0-1) stage,respectively(P<0.05 for all).The 3-year overall survival(OS)rate and progression free survival(PFS)rate of patients with low expression of lncRNA-p21 were lower than those with high expression of lncRNA-p21(38.78%vs 63.83%,8.16%vs 34.04%,P<0.05 for both).Cox proportional risk regression analysis showed that N stage[hazard ratio(HR)=1.881,95%confidence interval(CI):1.340-2.641,P<0.001]and low expression of lncRNA-p21(HR=0.609,95%CI:0.453-0.819,P=0.001)were associated with a poor prognosis in patients with lung adenocarcinoma.Conclusion The expression of lncRNA-p21 is down-regulated in lung adenocarcinoma tissue,and the low expression of lncRNA-p21 is associated with tumor size,differentiation degree,TN stage and poor prognosis.