负载氯己定的可注射水凝胶对粪肠球菌及生物膜的体外抑菌效果

Antibacterial Effect of Injectable Hydrogel with Chlorhexidine on Enterococcus Faecalis and Biofilm in Vitro

目的:研究负载氯己定的结冷胶复合材料体外抗粪肠球菌及生物膜的效果。方法:通过添加钙离子交联剂,制备含有纳米羟基磷灰石的结冷胶复合材料,并有效负载氯己定。通过对细菌生长曲线测定、扫描电镜等评价水凝胶体外抗粪肠球菌的效果,结晶紫染色定量和共聚焦激光扫描显微镜观察水凝胶对生物膜的作用。结果:随着水凝胶中氯己定浓度的增加,抗菌活性逐渐增强。当氯己定浓度为50μg/mL时,可显著抑制细菌生长及生物膜形成;当浓度为250μg/mL时,可表现出显著杀菌性。结论:负载氯己定的结冷胶复合材料抑制粪肠球菌生长及清除生物膜作用明显,有望作为新型可注射的根管消毒材料,促进顽固性根尖周炎的愈合。

Objective:To study the effect of chlorhexidine-loaded gellan gum hydrogel against Enterococcus faecalis and biofilm in vitro.Methods:The gellan gum containing hydroxyapatite was prepared by adding crosslinking agent(calcium ion),and chlorhexidine was effectively loaded.The effect of hydrogels against Enterococcus faecalis in vitro was evaluated by measuring bacterial growth curves and scanning electron microscopy,and their inhibiting the bacterial biofilms was observed by quantitative crystal violet staining and confocal laser scanning microscope.Results:As the concentration of chlorhexidine in the hydrogel increased,the antibacterial activity gradually increased.When the concentration of chlorhexidine was 50μg/mL,it could significantly inhibit bacterial growth and biofilm formation.When the concentration was 250μg/mL,it could show significant bactericidal effect.Conclusion:The gellan gum hydrogel loaded with chlorhexidine presented great effects on inhibiting the growth of Enterococcus faecalis and removing their biofilm.It might be a novel injectable biomaterial to disinfect root canals and promote the healing of refractory periapical periodontitis.