口腔扁平苔藓患者外周血iTr35细胞的分布比例及意义

Preliminary Study on Role of Inducible Regulatory T Cells 35 in Etiology of Oral Lichen Planus

目的:研究外周血诱导型调节性T细胞35(iTr35)和调节性T细胞(Treg)及白介素35(IL-35)的表达,探讨其在口腔扁平苔藓(OLP)发病机制中的作用。方法:选取2016年4月~2017年11月贵州医科大学附属医院确诊的OLP患者35例,同期25例年龄、性别匹配的健康志愿者为正常对照组,ELISA法检测外周血中IL-35水平,分析其与iTr35细胞的关系。上述收集病例中,选取OLP患者27例和正常对照组16例,采用流式细胞术检测外周血iTr35、Treg细胞比例,分析两种细胞的联系。结果:与正常对照组相比,OLP中Treg和iTr35细胞数量、IL-35表达水平显著升高,差异均有统计学意义(P<0.05);Spearman相关性分析显示,iTr35细胞与Treg细胞、CD4+Foxp3+Ebi3+p35+细胞(Treg细胞中产IL-35的T细胞)均存在正相关关系(r=0.385,P=0.048;r=0.723,P<0.001);而IL-35表达水平与iTr35细胞没有显著相关性(r=-0.173,P=0.454)。结论:OLP血清中高水平表达的IL-35为iTr35细胞的生成提供了可能,Treg细胞参与了iTr35细胞的生成,iTr35细胞比例升高可能在OLP发生、发展过程中起着重要作用。

Objective:To study the expression of inducible regulatory T cells 35(iTr35),regulatory T cells(Treg),and interleukin 35(IL-35)in peripheral blood,and to explore their role in the pathogenesis of oral lichen planus(OLP).Methods:A total of 35 OLP patients diagnosed in the Affiliated Hospital of Guizhou Medical University from April 2016 to November 2017 were selected.During the same period,25 healthy volunteers were selected as normal control group.The level of IL-35 in peripheral blood was detected by ELISA.Its relationship with iTr35 cells was analyzed.Among the cases collected above,27 OLP patients and 16 normal controls were selected.Flow cytometry was used to detect the proportion of peripheral blood iTr35 and Treg cells,and the relationship between two cells was analyzed.Results:Compared with the normal control group,the number of Treg and iTr35 cells and the expression level of IL-35 in OLP were significantly increased,and the differences were statistically significant(P<0.05).Spearman correlation analysis showed that iTr35 cells were positively correlated with Treg cells(r=0.385,P=0.048)and CD4Foxp3Ebi3p35 cells(T cells that produced IL-35 in Treg cells,r=0.723,P<0.001).While the expression level of IL-35 was not correlated with iTr35 cells(r=-0.173,P=0.454).Conclusion:The high level of IL-35 expressed in OLP serum provides the possibility for the generation of iTr35 cells.Treg cells participate in the generation of iTr35 cells.The increased proportion of iTr35 cells may play an important role in the occurrence and development of OLP.