温度和盐度对扇贝“渤海红”稚贝存活和生长的互作效应

Interaction Effects of Temperature and Salinity on Survival and Growth of Juvenile Scallop“Bohai Red”

使用中心复合试验设计与响应曲面分析法研究温度(16~28℃)和盐度(22~34)两个因素对扇贝“渤海红”稚贝存活和生长的互作效应。试验结果显示,温度的一次效应、二次效应和盐度的二次效应对扇贝“渤海红”稚贝的存活率、壳长增长率和体质量增长率的影响达到极显著水平(P<0.01);盐度的一次效应、温度和盐度的互作效应对扇贝“渤海红”稚贝的存活率达到显著水平(P<0.05),但对稚贝的壳长增长率与体质量增长率的影响不显著(P>0.05)。存活率、稚贝的壳长增长率与体质量增长率的回归模型的相关系数分别为0.9696、0.9969、0.9966,校正系数分别为0.9391、0.9938、0.9932,预测系数分别为0.7622、0.9726、0.9662。3个模型均有较好的拟合度,可用于预测扇贝“渤海红”稚贝的存活率、壳长增长率和体质量增长率。经过软件优化,得到温度和盐度的共同影响下存活率、壳长增长率和体质量增长率的最优结果:温度22.65℃、盐度28.51时,存活率为95.33%,壳长增长率197.50μm/d,体质量增长率21.58 mg/d,其可靠性为0.975。

The central composite experimental design and response surface analysis were used to study the combined effect of temperature(16—28℃)and salinity(22—34)on survival and growth of juvenile scallop“Bohai Red”.The results showed that the linear and quadratic effects of temperature and quadratic effects of salinity on juvenile survival rate,and the growth rates of shell length and body weight were very significant(P<0.01).The liner effects of salinity,the interaction effect of temperature and salinity on juvenile survival rate were significant(P<0.05),without significant on the growth rates of shell length and body weight of juveniles(P>0.05).By response surface analysis,a model equation was established for the relationship of juvenile survival rate,the growth rates of shell length and body weight to the two factors,with r^(2) of 0.9696,0.9969 and 0.9966,correlation coefficients of 0.9391,0.9938 and 0.9932 and prediction coefficients of 0.7622,0.9726 and 0.9662,respectively.The three models had a good fit,which was used to predict the survival rate,the growth rates of shell length and body weight of juveniles of scallop"Bohai Red".After software optimization,the suitable model under the combined influence of temperature and salinity was obtained.The best results of the growth rates of shell length and body weight were observed temperature of 22.65℃,and salinity of 28.51,with survival rate of 95.33%,and the shell length growth rate of 197.50μm/d,the body weight growth rate of 21.58 mg/d and reliability of 0.975.