PVDF中空纳米纤维的制备及其固定化酶的性能研究

Study on the Preparation of PVDF Hollow Nanofibers and the Performance of Immobilized Enzymes

利用同轴静电纺丝技术,制备了表面多孔的聚偏氟乙烯(PVDF)中空纳米纤维膜,并通过在内芯纺丝液中添加多巴胺,在固定化过程中多巴胺原位聚合生成聚多巴胺,将漆酶和辣根过氧化物酶固定在PVDF中空纳米纤维的内空腔中。通过优化同轴静电纺丝参数,探讨了外壳纺丝液中PVDF良溶液和不良溶液的溶剂比、纺丝温度、干燥条件对PVDF中空纳米纤维膜载体结构的影响,测试了固定化酶的中空纳米纤维的表面亲疏水性、酶的催化活性和稳定性,并分析了结构对固定化酶性能的影响。结果显示固定化酶活性保持了游离酶的80%;储存7 d后固定化酶的活性仍为初始活性的82%;进行10个反应操作后仍保持其初始活性的40%以上。研究结果表明同轴静电纺丝法制备的表面多孔的PVDF中空纳米纤维膜是酶固定化的优良载体。

A porous polyvinylidene fluoride(PVDF)hollow nanofiber membrane was prepared by using coaxial electrospinning technology,and by adding dopamine(DA)to the inner core spinning solution,DA was in-situ polymerized to form polydopamine(PDA)during the immobilization process,therefore laccase and horseradish peroxidase were fixed in the inner cavity of PVD hollow nanofibers.By optimizing the coaxial electrospinning parameters,the influence of the solvent ratio of the PVDF good solution and poor solution in the shell spinning solution,spinning temperature,and drying conditions on the structure of the PVDF hollow nanofiber membrane carrier was discussed.The surface hydrophilicity/hydrophobicity of the nanofibers,the catalytic activity and stability of the enzyme,and the influence of the structure on the performance of the immobilized enzyme were analyzed.The results show that the activity of the immobilized enzyme maintained 80%that of the free enzyme;after 7 days of storage,the activity of the immobilized enzyme was still 82%of the original activity;after 10 reaction operations,it still maintained more than 40%of its original activity.The porous PVDF hollow nanofiber membrane prepared by the coaxial electrospinning method is an excellent carrier for enzyme immobilization.