冬虫夏草菌固体发酵人参的工艺优化

A study on optimizing the fermentation process of ginseng by Chinese caterpillar fungus

目的优化冬虫夏草菌发酵人参药材的工艺。方法采用双向固体发酵技术,以冬虫夏草菌丝形成明显的菌蕾结构即为发酵终点,记录的发酵周期为响应值,药材粉碎粒度、基质含水量、CaCl_(2)浓度为自变量,在单因素实验基础上利用响应面法进行设计实验,筛选出冬虫夏草菌发酵人参的最佳工艺。结果最优的发酵工艺为药材粉碎粒度80目,基质含水量为28%,CaCl_(2)的加入量为206μmol/g,冬虫夏草菌丝体湿重与人参固体培养基干重的比例(m:m)=200%;按照以上条件接种后放入微生物培养箱中28℃避光培养,发酵周期为27d即可得到成熟的发酵人参产物。结论优化后的工艺稳定可行,重复性好,为后续发酵人参的研究提供了物质基础。

Objective To optimize the fermentation process of ginseng by Chinese caterpillar fungus.Methods The optimal fermentation process of ginseng by Chinese caterpillar fungus was identified by conducting designed experiments with the response surface methodology on the basis of single factor experiments,which applied the two-way solid state fermentation technology,viewed the formation of fungus buds as the experiment final goal,recorded the response value of a fermentation cycle,and took the granularity for grinded ginseng,the water content in fermentation matrix and the concentration of CaCl_(2) as the independent variables.Results The optimal fermentation process was as follows:The ginseng granularity was 80 mesh,the matrix water content was 28%,the concentration of CaCl_(2) was 206μmol/g,and the ratio of wet Chinese caterpillar fungus mycelium to dry ginseng solid medium(m:m)was 200%.After inoculation according to the above conditions,the mature fermented ginseng products can be obtained by placing them in a microbial incubator at 28℃ and cultivating them in the dark for 27 days.Conclusion The optimized process is stable and feasible,with a good repeatability,which provides a material basis for subsequent research on fermentation processes of ginseng.