半夏生物总碱对运动氧化应激大鼠胃黏膜的保护作用及机制研究

Protective Effect and Mechanism of Pinellia Total Alkaloids on Gastric Mucosa in Rats with Exercise Induced Oxidative Stress

目的探讨半夏生物总碱对运动氧化应激大鼠胃黏膜的保护作用及机制。方法将32只SD大鼠随机分为空白组、模型组、半夏生物总碱组(20 mg·kg^(-1))和阳性对照组(奥美拉唑,10 mg·kg^(-1)),每组8只。灌胃给药,每日1次,连续7 d。第7天灌胃给药1 h后,进行一次性负重力竭游泳运动造模。麻醉后处死所有大鼠,计算胃溃疡指数(UI),采用HE染色法观察胃黏膜组织病理形态;采用酶联免疫吸附法(ELISA)检测血清一氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)等氧化应激指标水平,以及胃黏膜组织胃泌素(GAS)、胃动素(MTL)含量。结果与空白组比较,模型组大鼠的UI值与胃黏膜组织GAS、MTL含量,以及血清NO、MDA含量均显著升高(P<0.01),血清SOD、GSH-Px水平显著降低(P<0.01)。与模型组比较,半夏生物总碱组大鼠的UI值与胃黏膜组织GAS、MTL含量,以及血清NO、MDA含量均显著降低(P<0.01),血清SOD、GSH-Px水平显著升高(P<0.01)。结论半夏生物总碱具有抗氧化作用,能纠正运动应激导致的胃黏膜组织GAS、MTL分泌异常状态,逆转运动氧化应激对胃黏膜的损伤,减少胃溃疡的发生。

Objective The purpose of this paper is to discuss the protective effect and mechanism of Pinellia total alkaloids on gastric mucosa in rats with exercise induced oxidative stress.Methods 32 healthy male SD rats were randomly divided into blank group(equal amount of normal saline),model group(equal amount of normal saline),Pinellia total alkaloids group(20 mg·kg^(-1))and positive control group(omeprazole,10 mg·kg^(-1)),with 8 rats in each group.Rats in each group were gavaged every morning for 7 days.In addition to blank group,model group,Pinellia total alkaloid group and positive control group were given one-time exhaustive swimming after intragastric administration for 1 hour on the 7 th day.All rats were killed after anesthesia,and their gastric ulcer indexes(UI)were calculated,and the morphology of rats’ gastric mucosa was observed under optical microscope after HE staining,and the levels of rats’ serum nitric oxide(NO),contents of malondialdehyde(MDA),the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and the levels of rats’ gastric mucosa gastrin(GAS)and motilin(MTL)were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the blank group,in model group,UI was significantly increased(P<0.01),gastric mucosa showed significant pathological changes,gastric GAS and MTL contents were significantly increased(P<0.01),serum NO and MDA contents were significantly increased(P<0.01),serum SOD and GSH Px activities were significantly decreased(P<0.01).Compared with the model group,the UI of Pinellia total alkaloid group rats was significantly decreased(P<0.01),the pathological morphology of gastric mucosa was significantly improved,the gastric GAS and MTL contents were significantly decreased(P<0.01),the serum NO and MDA contents were significantly decreased(P<0.01),and the serum SOD and GSH Px activities were significantly increased(P<0.01).Conclusion Pinellia total alkaloids showed antioxidant effect,and can correct the abnormal secretion of GAS and MTL caused by exercise stress,reverse the damage of gastric mucosa caused by exercise oxidative stress,protect gastric mucosa,and reduce the occurrence of gastric ulcer.