摘要
建立了应用固相萃取-超高效液相色谱-串联质谱技术(SPE-UPLC-MS/MS)同时测定水环境中包括磺胺类、四环素类、大环内酯类和喹诺酮类在内的4大类15种抗生素的方法。采集的水样中加入同位素替代物后通过HLB固相萃取柱进行富集浓缩,UPLC-MS/MS进行测定,并采用内标法定量。结果表明,15种抗生素在0.5~50μg/L线性范围内检出限为0.04~0.09 ng/L,定量限为0.16~0.36 ng/L,样品加标回收率为59.5%~102.8%,相对标准偏差(RSD)均小于12%。该方法适用于水环境中痕量残留的抗生素检测。
The method for simultaneous determination of 15 kinds of antibiotics in 4 categories including sulfonamides, tetracyclines, macrolides and quinolones in the water by solid-phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry(SPE-UPLC-MS/MS) technology was established. The collected water samples were enriched and concentrated by the HLB solid phase extraction column after adding isotope substitutes. UPLC-MS/MS was used for the determination and the internal standard method was used for quantification. The results showed that the detection limits of 15 antibiotics in the linear range of 0.5-50 μg/L were 0.04-0.09 ng/L, the limits of quantification were 0.16-0.36 ng/L, and the sample recoveries were 59.5%-102.8%. The relative standard deviations(RSDs) of samples were less than 12%. This method could be employed for the determination of trace antibiotic residues in the water environment.
作者
宋焕杰
谢卫民
王俊
陈晓飞
闫春淼
沙雪妮
刘红
李柏林
SONG Huanjie;XIE Weimin;WANG Jun;CHEN Xiaofei;YAN Chunmiao;SHA Xueni;LIU Hong;U Bolin(School of Resources and Environmental Engineering,Wuhan University of Technology,Wuhan 430070;Center for Environmental Monitoring and Scientific.Research of Changjiang River Basin Ecology and Environment Administration,Ministry of Ecology and Environment,Wuhan 430000;Wuhan Lingang Economic and Technological Development Zone Service Industry Development Investment Group Co.Ltd.,Wuhan 430040;Hubei Academy of Environmental Sciences,Wuhan 430070)
出处
《分析试验室》
CAS
CSCD
北大核心
2022年第1期50-54,共5页
Chinese Journal of Analysis Laboratory
基金
武汉市应用基础前沿项目(2018060401011313)资助。
关键词
抗生素
超高效液相色谱-串联质谱
检测方法
水环境
antibiotics
ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
detection method
water environment