筛选EV71 3C蛋白酶切割的宿主细胞蛋白

Screening Host Cell Proteins for EV71 3C Protease Cleavage

肠道病毒EV71型是引起儿童手足口病的主要病原体,3C是其编码的蛋白酶之一.3C在切割病毒前体蛋白为成熟蛋白的同时.切割一些具有重要功能的细胞蛋白,影响细胞的生理功能.为进一步了解EV71与宿主的关系,明确EV71致病机制的细节,在前期以3C蛋白为诱饵开展的酵母双杂交工作的基础上,选取了5种细胞蛋白,分析其是否是3C蛋白可能的切割底物.发现ZMYM2蛋白可以被3C切割:这一切割效应是依赖3C蛋白酶活性的特异性切割,无需RNA介导.上述发现为进一步揭示EV71致病机制提供了线索.

Enterovirus EV71 type is the main pathogen causing hand,foot and mouth disease(HFMD)and fatal neurological diseases in young children.EV71 infection induces cytopathogenesis through a variety of pathways,while the viral protease 3C plays an important role via cutting some important cellular proteins.To discover the cellular substrate of 3C,a yeast two-hybrid was employed to assay to screen for 3C-interacting proteins previously.Here,5 potential interact proteins were selected to analyze whether they were possible cutting substrates of 3C protein.It was found that ZMYM2 protein could be cleaved by 3C,and this cleavage effect depended on the specific cleavage of 3C protease activity without RNA mediation.These findings provide clues to further reveal the pathogenesis of EV71.