高效液相色谱法测定化妆品中依克多因的含量

Determination of the content of ectoine in cosmetics by high performance liquid chromatography

建立高效液相色谱法测定化妆品中依克多因的分析方法,采用Agilent Poroshell 120 EC-C;色谱柱(100 mm×3.0 mm,2.7μm)分离,以甲醇和p H为3.0的40 mmol/L磷酸二氢钠-10 mmol/L 1-庚烷磺酸钠缓冲溶液梯度洗脱,流速0.8 m L/min,柱温30℃,检测波长210 nm。采用外标法定量测定化妆品中的依克多因含量。结果表明,依克多因在5~800 mg/L的质量浓度范围内呈现良好线性关系,相关系数为0.999 8,方法的检出限和定量限分别为0.3和1.0 mg/L。该方法具有分离效率高、分析时间短、节省溶剂等优点,解决了依克多因在C;色谱柱上保留弱的问题。

A method for the determination of ectoine in cosmetics by high performance liquid chromatography (HPLC) was established.Agilent Poroshell 120 EC-C;column (100 mm×3.0 mm,2.7μm)was used for sample analysis.Methanol and 40 mmol/L sodium dihydrogen phosphate-10 mmol/L sodium 1-heptanesulfonate solution (adjusted p H to 3.0 by phoshporic acid) were used as mobile phases for gradient elution,with a flow rate of 0.8 m L/min.The temperature of the column was 30℃.Samples were quantitated by diode array detector at 210 nm with external standard method.Result shows that the standard working curve of ectoine has good linear relationship in the range of 5-800 mg/L.The limit of detection and quantitation is 0.3 and 1.0 mg/L,respectively.The method has the advantages of high separation efficiency,short analysis time,and solvent cost saving.It solves the problem of ectoine retaining on C;chromatography column.