酶抑制-比色法农残速测试剂盒检测蔬菜农药残留的结果分析

Analysis of pesticide residues in vegetables by enzyme inhibition colorimetric method

为了解商品化酶抑制-比色法试剂盒的检测效果,将6个品牌的试剂盒检测蔬菜中农药残留的结果和用色谱、质谱检测其中50种有机磷及10种氨基甲酸酯类农药的结果进行比对分析,根据不同的判定阈值对试剂盒检测结果进行评价,得出各试剂盒的假阳性率、假阴性率和符合率,由此对商品化酶抑制-比色法试剂盒的检测效果进行比对分析。结果表明:在此6个品牌的试剂盒中D试剂盒的检测效果最佳,在所设定的7个判定阈值下,其符合率均最高;商品化的酶抑制-比色法农残速测试剂盒的检测效果同阳性样品的判定阈值存在一定的关系,假阳性率随着判定阈值的提高而降低,假阴性率随着判定阈值的提高而增加,但同色谱、质谱检测的符合率都未能达到100%;当判定阈值设为20%时效果最好,有3个品牌的试剂盒的检测效果同色谱、质谱检测结果的符合率达到最高,且在参与比对的6个试剂盒中没有试剂盒在此判定阈值下出现最低的符合率。因此,建议对国标和行标中的判定阈值进行修改。

Objective To understand the detection effect of commercial enzyme inhibition colorimetric kit.Methods Six brands of kits were used to detect pesticide residues in vegetables.The detection results were compared with those of 50 kinds of organophosphorus pesticides and 10 kinds of carbamate pesticides detected by chromatography and mass spectrometry,according to different thresholds, the test results of the kits were evaluated, and the false positive rate, false negative rate and coincidence rate of each kit were obtained.The test results of commercial enzyme inhibition colorimetric kit were compared and analyzed.Results The detection effect of D kit was the best among the six brands of kits, and the coincidence rate was the highest under the seven thresholds;There is a certain relationship between the detection effect of commercial enzyme inhibition colorimetric pesticide residue test kit and the determination threshold of positive samples.The false positive rate decreases with the increase of the determination threshold, and the false negative rate increases with the increase of the determination threshold, but the coincidence rate with chromatography and mass spectrometry cannot reach 100%;When the threshold is set to 20%, the effect is the best.The coincidence rate of three brands of kits with the results of chromatography and mass spectrometry is the highest, and none of the six kits involved in the comparison has the lowest coincidence rate under this threshold.Conclusion The threshold values in National Standard and Trade Mark is suggested to modify.