摘要
RRTZF基因在植物的生长发育和逆境胁迫中起着重要作用。OsTZF9基因是水稻RRTZF基因家族的一员,为分析OsTZF9基因的生物学功能,构建了由Ubiqutin启动子驱动的植物过表达载体pCXUN-TZF9,通过农杆菌介导法获得了转基因再生植株6个克隆。进一步分析转基因植株后代的表型发现,与野生型日本晴相比,OsTZF9基因过表达植株的株高、分糵数、有效穗数、穗长和千粒重均下降;与转基因阴性分离株相比,OsTZF9基因过表达植株抽穗期推迟。
RR-TZF gene plays an important role in plant growth,development and stress responses.OsTZF9 gene is a member of rice RR-TZF gene family.In order to analyze the biological function of OsTZF9 gene,a plant overexpression vector pCXUN-TZF9 driven by Ubiqutin promoter was constructed,and 6 clones of transgenic regenerated plants were obtained by using the agrobacterium-mediated method.Further analysis of the phenotype of the progenies of the transgenic plants showed that plant height,tiller number,effective panicle number,panicle length and 1000-grain weight of the OsTZF9-overexpressed plants were decreased compared with those of the wild-type Nipponbare;compared with the transgenic negative isolated plants,heading date of the OsTZF9-overexpressed plants was delayed.
作者
周淑芬
刘宝
张迪
ZHOU Shu-fen;LIU Bao;ZHANG Di(Institute of Biological Technology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;Fujian Key Laboratory of Agricultural Genetic Engineering, Fuzhou, Fujian 350003, China)
出处
《福建农业科技》
CAS
2021年第11期1-6,共6页
Fujian Agricultural Science and Technology
基金
福建省科技计划项目——省属公益类科研院所基本科研专项(2017R1019-3)。
