外源水杨酸诱导油菜幼苗响应干旱胁迫的生理机制

Physiological Mechanism of Exogenous Salcylic Acid Alleviates Drought Stress on Seedlings of Brassica napus L.

[目的]探索外源水杨酸诱导油菜幼苗响应干旱胁迫的生理机制。[方法]以油菜幼苗为材料,采用15%的PEG-6000处理幼苗模拟干旱胁迫,利用0.1 mmol/L外源水杨酸对叶片进行喷施,探讨不同干旱程度下水杨酸对油菜叶片抗氧化系统酶活、丙二醛含量以及脯氨酸含量的影响。[结果]在不同干旱程度下,0.1 mmol/L的水杨酸处理可显著降低叶片中MDA的含量,增加叶片中抗氧化系统酶活以及脯氨酸含量。[结论]0.1 mmol/L的水杨酸处理可有效调控油菜幼苗响应干旱胁迫,缓解干旱胁迫导致的膜脂过氧化等危害的发生。

[Objective]To explore the physiological mechanism of exogenous salicylic acid inducing rape seedlings to respond to drought stress.[Method]Using rape seedlings as materials,15%PEG-6000 was used to treat the seedlings to simulate drought stress,and 0.1mmol/L exogenous salicylic acid was used to sprayed on the leaves to explore the effects of salicylic acid on the enzyme activities of the antioxidant system,malondialdehyde content and proline content of rape leaves under different drought levels.[Result]Under different drought degrees,0.1 mmol/L salicylic acid treatment could significantly reduce the MDA content in leaves,and increase the enzyme activity of antioxidant system and proline content in leaves.[Conclusion]Salicylic acid treatment of 0.1 mmol/L can effectively alleviate the drought stress and protect the normal physiological and biochemical metabolism of the seedlings for Brassica napus L..