多囊卵巢综合征患者血清miR-155、IL-6水平与IVF-ET妊娠结局的关系

The Relationship Between Serum MiR-155,IL-6 Levels and IVF-ET Pregnancy Outcome in Patients with Polycystic Ovary Syndrome

目的探究多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者血清miR-155、白细胞介素6(interleukin-6,IL-6)水平与体外受精-胚胎移植(IVF-ET)助孕结局的相关性。方法选取2017年5月至2021年1月在我院接受IVF-ET治疗的150例PCOS不孕患者作为研究对象。根据临床妊娠结局分为妊娠组(n=68)及未妊娠组(n=82)。收集两组患者一般资料,酶联免疫吸附试验检测IL-6水平,实时荧光定量PCR法检测血清miR-155水平。Spearman法分析血清IL-6、miR-155水平与睾酮(T)水平、成熟卵率、受精率、优质胚胎率的相关性。多因素Logistic分析影响IVF-ET妊娠结局的因素。结果妊娠组患者血清IL-6、miR-155及T水平低于未妊娠组(均P<0.001)。妊娠组获卵数、成熟卵率、受精率及优质胚胎率均高于未妊娠组,差异具有统计学意义(均P<0.001)。IL-6、miR-155水平与T水平呈正相关,与成熟卵率、受精率、优质胚胎率呈负相关(均P<0.001),IL-6、miR-155表达水平呈正相关(r=0.502,P<0.001)。IL-6、miR-155、T是影响PCOS患者IVF-ET妊娠结局的独立危险因素,优质胚胎率是保护因素(均P<0.05)。结论PCOS患者IVF-ET助孕妊娠成功者血清miR-155、IL-6低表达,与IVF-ET结局指标关系密切,是独立的危险因素。

Objective To investigate the correlation between serum levels of miR-155,interleukin-6(IL-6)and the outcome of in vitro fertilization embryo transfer(IVF-ET)in patients with polycystic ovary syndrome(PCOS).Methods 150 PCOS infertile patients who received IVF-ET treatment in our hospital from May,2017 to January,2021 were selected for the study.These patients were divided into the pregnancy group(n=68)and non-pregnancy group(n=82)according to pregnancy outcome.The general data of the two groups were collected.The level of IL-6 was detected by enzyme-linked immunosorbent assay,while the level of serum miR-155 was detected by real-time fluorescent quantitative PCR.Spearman method was applied to analyze the correlation between serum IL-6,miR-155 levels and testosterone(T),mature egg rate,fertilization rate,and high-quality embryo rate.Multivariate logistic analysis was conducted to analyze the influencing factors of IVF-ET pregnancy outcome.Results Serum levels of IL-6,miR-155 and T in the pregnancy group were lower than those in the non-pregnancy group(all P<0.001).The number of retrieved eggs,mature egg rate,fertilization rate and high-quality embryo rate in the pregnancy group were higher than those in the non-pregnancy group(all P<0.001).The levels of IL-6 and miR-155 were positively correlated with T level,while negatively correlated with mature egg rate,fertilization rate and high-quality embryo rate(all P<0.001).The expression levels of IL-6 and miR-155 were positively correlated(r=0.502,P<0.001).IL-6,miR-155 and T were independent risk factors for IVF-ET pregnancy outcome in PCOS patients,while high-quality embryo rate was a protective factor(all P<0.05).Conclusion The low expression of serum miR-155 and IL-6 in PCOS patients with successful IVF-ET is closely related to the outcome of IVF-ET and is an independent risk factor.