TOP2A在肺腺癌中的表达及其与疾病预后的关系研究

The Expression of TOP2A in Lung Adenocarcinoma and Its Relationship with Disease Prognosis

目的探讨拓扑异构酶Ⅱ(TOP2A)在肺腺癌中的表达情况,并分析其与患者预后的关系。方法纳入我院2018年1月至2020年1月收治的肺腺癌患者98例,采用免疫组化法检测肺腺癌组织、癌旁组织中TOP2A表达情况,分析肺腺癌组织中TOP2A表达与临床特征的关系。根据TOP2A表达情况分成TOP2A高表达组、TOP2A低表达组,比较两组预后情况(即总生存率),利用Cox多因素回归模型分析患者预后的影响因素。结果肺腺癌组织中TOP2A高表达率为66.33%,高于癌旁组织的17.35%,且TOP2A高表达组临床分期Ⅲ期、淋巴结转移占比分别为44.62%、46.15%,高于TOP2A低表达组的12.12%、9.09%(P<0.05)。TOP2A高表达组总生存率为72.31%,低于TOP2A低表达组的90.91%(P<0.05)。Cox多因素分析提示年龄≥60岁、临床分期Ⅲ期、淋巴结转移、TOP2A高表达是增加肺腺癌患者死亡风险的影响因素,术后放疗是降低死亡风险的保护性因素(P<0.05)。结论肺腺癌患者癌组织中TOP2A呈高表达,且TOP2A高表达会增加患者死亡风险,临床需引起重视。

Objective To explore the expression of topoisomeraseⅡ(TOP2A)in lung adenocarcinoma,and to analyze its relationship with the prognosis of patients.Methods 98 patients with lung adenocarcinoma admitted to our hospital from January,2018 to January,2020 were included in the study.Immunohistochemical method was conducted to detect the expression of TOP2A in lung adenocarcinoma tissues and adjacent tissues,and we analyzed the relationship between TOP2A expression in lung adenocarcinoma tissues and clinical features.According to TOP2A expression,these patients were divided into the TOP2A high expression group and TOP2A low expression group.The prognosis(overall survival rate)of the two groups were compared,and COX multivariate regression model was applied to analyze the factors affecting the prognosis of patients.Results The high expression rate of TOP2A in lung adenocarcinoma tissue was 66.33%,which was higher than 17.35%in adjacent tissues.In addition,the clinical stage III and lymph node metastasis percentages in the TOP2A high expression group were 44.62%and 46.15%,respectively,which were higher than those in the TOP2A low expression group with 12.12%and 9.09%,respectively(P<0.05).The overall survival rate of the TOP2A high expression group was 72.31%,which was lower than 90.91%of the TOP2A low expression group(P<0.05).Cox multivariate analysis showed that age≥60 years old,clinical stage III,lymph node metastasis and high expression of TOP2A were the risk factors of death in patients with lung adenocarcinoma,while postoperative radiotherapy was the protective factor to reduce the risk of death(P<0.05).Conclusion TOP2A is highly expressed in cancer tissues of patients with lung adenocarcinoma,and the high expression of TOP2A can increase the risk of death in patients,which requires clinical attentions.