人参皂苷Rg3调控Dickkopf相关蛋白3对肝癌细胞增殖和凋亡的影响

The Effect of Ginsenoside Rg3 Regulateing Dickkopf-Related Protein 3 on the Proliferation and Apoptosis of Hepatocellular Carcinoma Cells

目的:探究人参皂苷Rg3对肝癌细胞增殖的影响及其作用机制。方法:以人肝癌细胞系HepG2为研究对象,分为对照组(Control)、人参皂苷Rg3组(G-Rg3)、人参皂苷Rg3+Dickkopf相关蛋白3无关片段组(G-Rg3+DKK3-NC)以及人参皂苷Rg3+Dickkopf相关蛋白siRNA组(G-Rg3+DKK3-siRNA)。其中G-Rg3组细胞给予G-Rg3(40mg/L)干预48h;G-Rg3+DKK3-NC组细胞转染DKK3-NC 24h,后G-Rg3干预48h;G-Rg3+DKK3-siRNA组细胞转染DKK3-siRNA后,G-Rg3干预48h;Control组细胞正常培养。MTT法检测细胞增殖;流式细胞术检测细胞凋亡;蛋白质免疫印迹法检测DKK3、Wnt、β-连环蛋白(β-catenin)表达。结果:与Control组相比,G-Rg3组细胞凋亡指数显著升高(P<0.01)、细胞增殖显著降低(P<0.01)、DKK3表达显著升高(P<0.01),Wnt和β-catenin表达均显著降低(P<0.01);与G-Rg3组相比,G-Rg3+DKK3-siRNA组细胞凋亡指数显著降低(P<0.01)、细胞增殖显著增加(P<0.01)、DKK3表达显著降低(P<0.01),Wnt和β-catenin表达均显著升高(P<0.05或P<0.01)。结论:G-Rg3能够抑制肝癌细胞增殖,促进凋亡,其机制与促进DKK3表达,抑制Wnt/β-catenin信号传导相关。

Objective:To explore the effect of ginsenoside Rg3 on the proliferation of liver cancer cells and its mechanism.Methods:The human liver cancer cell line Hep G2 was used as the research subject which was divided into control group(Control),ginsenoside Rg3 group(G-Rg3),ginsenoside Rg3+Dickkopf-related protein 3 irrelevant fragment group(G-Rg3+DKK3-NC),and ginsenoside Rg3+Dickkopf-related protein siRNA group(G-Rg3+DKK3-siRNA).G-Rg3 group cells were given G-Rg3(40 mg/L)intervention for 48 h;24h after G-Rg3+DKK3-NC group cells were transfected with DKK3-NC,G-Rg3 intervention was conducted 48 h;after G-Rg3+DKK3-siRNA group cells were transfected,G-Rg3 intervention was conducted 48 h;cells in the control group were cultured normally.MTT method was used to detect cell proliferation;flow cytometry was used to detect cell apoptosis;Western blotting was used to detect the expression of DKK3,Wnt,andβ-catenin.Results:Compared with the control group,the apoptosis index of G-Rg3 group was significantly increased(P<0.01),cell proliferation was significantly decreased(P<0.01),DKK3 expression was significantly increased(P<0.01),Wnt andβ-catenin expression were significantly decreased(P<0.01);compared with the G-Rg3 group,the apoptosis index in the G-Rg3+DKK3-siRNA group was significantly reduced(P<0.01),cell proliferation was significantly increased(P<0.01),DKK3 expression significantly decreased(P<0.01),and the expression of Wnt andβ-catenin increased significantly(P<0.05 or P<0.01).Conclusion:G-Rg3 can inhibit the proliferation of liver cancer cells and promote apoptosis.The mechanism is related to the promotion of DKK3 expression and inhibition of Wnt/β-catenin signal transduction.