环巴胺对前列腺增生症大鼠细胞增殖与凋亡的影响

Effects of Cyclopamine on Cell Proliferation and Apoptosis in Rats with Benign Prostatic Hyperplasia

目的:观察环巴胺对前列腺增生症大鼠细胞增殖与凋亡的影响。方法:将30只BPH大鼠随机分为模型组(BPH组)和环巴胺组(Cyclopamine组),未造模的15只大鼠为正常对照组(Normal组)。Cyclopamine组大鼠腹腔注射环巴胺,Normal组和BPH组腹腔注射等剂量生理盐水,1次/d,连续7d。给药结束后处死大鼠,获得各组大鼠的前列腺组织,并对其进行各项指标的测定。结果:与Normal组相比,BPH组和Cyclopamine组的前列腺湿重、体积及前列腺指数显著升高(P<0.05);腺上皮呈乳头状增生,腺体数量增多,腺腔变大,间质和血管扩张充血水肿;前列腺细胞凋亡小体表达率明显降低(P<0.05),细胞增殖速率明显增高(P<0.05)。与BPH组相比,Cyclopamine组前列腺湿重、体积及前列腺指数显著降低[(3.18±0.28)比(2.03±0.22);(2.30±0.26)比(1.45±0.27);(0.78±0.23)比(0.46±0.17)](P<0.05);前列腺组织病理学改变改善明显;凋亡小体表达率明显上升[(3.17±0.68)比(5.25±0.77)](P<0.05);间质、上皮细胞增殖速率明显降低[吸光度4d(1.35±0.09)/(1.62±0.17)比(0.94±0.11)/(1.26±0.15);5d(2.24±0.16)/(2.73±0.27)比(1.74±0.17)/(2.16±0.20)、细胞数目4d(21.57±1.89)/(31.32±2.54)比(17.88±1.26)/(25.44±2.39);5d(33.66±3.16)/(44.97±4.29)比(29.14±2.42)/(39.75±3.82)](P<0.05)。结论:环巴胺能够抑制前列腺增生症大鼠前列腺上皮细胞和间质细胞的增殖,促进细胞凋亡的发生。

Objective:To observe the effect of Cyclopamine on cell proliferation and apoptosis in rats with benign prostatic hyperplasia.Methods:Thirty BPH rats were randomly divided into model group(BPH group)and Cyclopamine group(Cyclopamine group),and 15 rats without modeling served as normal control group(Normal group).Rats in Cyclopamine group were intraperitoneally injected with Cyclopamine,and rats in normal group and BPH group were intraperitoneally injected with the same dose of normal saline,once a day,for 7 consecutive days.After the administration,the rats were killed to obtain the prostate tissue of each group,and the indexes were determined.Results:Compared with normal group,the wet weight,volume and prostate index of BPH group and Cyclopamine group were significantly increased(P<0.05).The glandular epithelium showed papillary hyperplasia,and the number of glands increased,the glandular cavity enlarged,the stroma and blood vessels dilated with hyperemia and edema.The expression rate of apoptotic bodies was significantly decreased(P<0.05),and the proliferation rate was significantly increased(P<0.05).Compared with BPH group,the wet weight,volume and prostate index of Cyclopamine group were significantly lower[(3.18±0.28)g vs(2.03±0.22)g;(2.30±0.26)ml vs(1.45±0.27)ml;(0.78±0.23)%vs(0.46±0.17)%](P<0.05).The histopathological changes of prostate were improved significantly.The expression rate of apoptotic bodies increased significantly[(3.17±0.68)%vs(5.25±0.77)%](P<0.05).The proliferation rate of stromal and epithelial cells decreased significantly[OD 4d(1.35±0.09)/(1.62±0.17)vs(0.94±0.11)/(1.26±0.15);5d(2.24±0.16)/(2.73±0.27)vs(1.74±0.17)/(2.16±0.20);cell number 4d(21.57±1.89)/(31.32±2.54)vs(17.88±1.26)/(25.44±2.39);5d(33.66±3.16)/(44.97±4.29)vs(29.14±2.42)/(39.75±3.82)](P<0.05).Conclusion:Cyclopamine can inhibit the proliferation of prostatic epithelial cells and stromal cells,and promote the occurrence of apoptosis.