CD14启动子-159位点基因多态性与中国朝鲜族2型糖尿病足患者足背动脉硬化相关性研究

Study on the relationship between gene polymorphism of-159 locus in CD14 gene promoter and foot dorsal arteriosclerosis in Chinese Korean type 2 diabetic foot patients

目的研究CD14基因-159位点基因多态性与中国朝鲜族2型糖尿病足患者(DF)足背动脉硬化(FDA)的关联性。方法选取2018年10月至2020年5月在延边大学附属医院内分泌科及手足外科住院的朝鲜族DF患者286例,彩色多普勒超声观察患者FDA情况,将存在FDA的患者分为无溃疡组(NFDAU组)和有溃疡组(FDAU组),记录全部2型糖尿病(Type 2 diabetic mellitus,T2DM)患者血液生化指标。同时,应用聚合酶链反应-限制性片段长度多态性技术对所有DF患者的CD14基因-159位点进行基因型分析。结果无溃疡组和有溃疡组CD14基因-159位点上,各基因型及等位基因频率比较,差异无统计学意义(χ^(2)=1.801,P>0.05;χ^(2)=1.864,P>0.05);CC基因与CT及TT基因基因频率比较,差异有统计学意义(χ^(2)=0.009,P<0.05);NFDAU组和FDAU组在病程、吸烟饮酒、高血糖及高尿酸指标方面比较,差异有统计学意义(P<0.05);Logistic回归分析显示,病程、吸烟饮酒及C等位基因均与FDAU相关联(P<0.05)。结论CD14基因-159位点基因多态性与中国朝鲜族DF并发FDA相关,其中CC纯合子基因是DF患者并发FDAU的遗传学风险因素。病程、吸烟、饮酒及C等位基因是FDAU的危险因素。

Objective To study the association between gene polymorphism of-159 locus in CD14 gene promoter and foot dorsal arteriosclerosis(FDA)in Chinese Korean type 2 diabetic foot patients(DF).Methods A total of 286 Korean DF patients hospitalized in the Department of Endocrinology and Department of Hand and Foot Surgery in Yanbian University Hospital from October 2018 to May 2020 were selected.The FDA status of the patients was observed using color Doppler ultrasound.The patients with FDA were classified as the non-ulcer group(NFDAU)and the ulcer group(FDAU).All biochemical blood indicators of type 2 diabetic mellitus(T2DM)patients were recorded.At the same time,the polymerase chain reaction-restriction fragment length polymorphism technique was used to perform genotype analysis on the-159 locus of CD14 gene in all DF patients.Results There was no statistically significant difference in the genotypes and allele frequencies at-159 locus of CD14 gene between the non-ulcer group(NFDAU)and the ulcer group(FDAU)(χ^(2)=1.801,P>0.05;χ^(2)=1.864,P>0.05).The gene frequency difference between CC,CT and TT genes was statistically significant(χ^(2)=0.009,P<0.05).There were significant differences in disease course,smoking and drinking,hyperglycemia,and hyperuric acid indicators between NFDAU and FDAU(P<0.05).Logistic regression analysis showed that the course of disease,smoking and drinking,and C allele were all associated with FDAU(P<0.05).Conclusion The polymorphism of 159 locus of CD14 gene is associated with FDA in Chinese Koreans with DF.The CC homozygous gene is a genetic risk factor for DF patients with FDAU.The course of disease,smoking,drinking and C allele are risk factors for FDAU.