降钙素基因相关肽对高氧肺损伤的Notch通路调控

Calcitonin gene-related peptide regulates Notch pathway in hyperoxia induced lung injury

目的研究降钙素基因相关肽(CGRP)对早产鼠高氧肺损伤的Notch通路调控作用。方法将SD早产鼠分为空气组、高氧组、高氧联合CGRP组、高氧联合CGRP和CGRP8-37组及高氧联合CGRP和MW167组。CGRP8-37为CGRP拮抗剂,MW167为Notch信号通路阻断剂。分别于第3、7、14天观察和比较5组早产鼠肺组织病理变化及肺损伤病理评分,并应用实时定量聚合酶链反应(Q-PCR)法检测各组早产鼠肺组织Notch 1、Hes、heRP mRNA表达情况。结果与高氧组、高氧联合CGRP和CGRP8-37组、高氧联合CGRP和MW167组比较,高氧联合CGRP组早产鼠第3、7、14天肺损伤病理评分均明显降低,Notch 1、Hes、heRP mRNA表达水平均明显升高,差异均有统计学意义(P<0.05)。结论CGRP可能通过Notch通路促进其信号表达,对早产鼠高氧肺损伤发挥保护效应。

Objective To study the regulatory effect of calcitonin gene-related peptide(CGRP)on Notch signaling pathway in premature rats with hyperoxia lung injury.Methods SD premature rats were divided into the air group,hyperoxia group,hyperoxia+CGRP group,hyperoxia+CGRP8-37 group and hyperoxia+CGRP+MW167 group.The pathological changes of lung tissue and pathological scores of lung injury on 3,7,14 d were observed and compared among 5 groups,and the mRNA expressions of Notch 1,Hes and heRP in lung tissue were detected by Q-PCR.Results The pathological scores of lung injury on 3,7,14 d in the high oxygen+CGRP group were significantly decreased compared with those in the high oxygen group,high oxygen+CGRP+CGRP8-37 group and high oxygen+CGRP+MW167 group(P<0.05),while the mRNA expression levels of Notch 1,Hes and heRP were significantly increased,and the differences were statistically significant(P<0.05).Conclusion CGRP may play a protective role in hyperoxia induced lung injury in premature rats by promoting its signal expression possibly through Notch pathway.