枯草芽孢杆菌表面展示技术用于黏膜疫苗的研究进展

Research progress on Bacillus subtilis surface display technology for mucosal vaccine

枯草杆菌全名枯草芽孢杆菌(Bacillus subtilis),因其优秀的益生特性及芽孢良好的抗逆性而备受研究者青睐,由于芽孢的特殊结构及独特的生理特性,是酶和免疫原等外源蛋白的理想锚定点。采用枯草杆菌进行芽孢表面展示被认为是表达高活性和高稳定性的外源蛋白的方法之一。本文主要对枯草杆菌芽孢表面展示抗原蛋白以生产黏膜疫苗的策略和应用前景进行综述。

Bacillus subtilis was favored by researchers attributing to the excellent probiotic properties and strong stress resistance of spores.Owing to the special structure and unique physiological characteristics,the spores are an ideal place for foreign proteins such as enzymes and immunogens to anchor.B.subtilis spore surface display is one of the methods to express foreign proteins with high activity and stability.This article mainly reviewed the strategies and application prospects of displaying antigen proteins on the surface of B.subtilis spores to produce mucosal vaccines.