次氯酸钠处理种子对芹菜幼苗可溶性糖含量及相关基因表达的影响

Effects of seed-soaking with sodium hypochlorite on soluble sugar content and expression profiles of related genes of celery seedlings

种皮革质化、透水性差的特点导致芹菜种子发芽慢、发芽不整齐。次氯酸钠溶液作为消毒剂可以促进种子萌发,但次氯酸钠处理种子对芹菜幼苗的影响尚不清楚。本文探讨了不同浓度次氯酸钠溶液浸种对芹菜3个生长阶段(播种后20、40、60 d)幼苗可溶性糖含量的影响,并结合RT-qPCR技术分析了与可溶性糖代谢通路相关基因表达情况(蔗糖合成酶基因SS、蔗糖磷酸合成酶基因SPS、尿苷二磷酸葡萄糖焦磷酸化酶基因UGPase、蔗糖磷酸磷酸酶基因SPP、转化酶基因Ivr)。研究发现5%的次氯酸钠溶液浸种可提高芹菜幼苗叶片可溶性糖含量且效果最佳,其中‘六合黄心芹’和‘赛雪’芹菜幼苗叶片在播种后60 d的含糖量分别为13.20和14.00 mg·g^(-1) (FW);此外,两个品种三个生长阶段的可溶性糖合成相关基因SS和SPS的相对表达量均在5%次氯酸钠浸种最高。结果表明5%次氯酸钠溶液浸泡芹菜种子是提高幼苗可溶性糖含量的适合浓度,可为提高芹菜产量提供理论依据。

The leather-like characteristics and low permeability of seed coat lead to slow and irregular germination of celery seeds. Sodium hypochlorite solution as a disinfectant can promote seed germination,but the effects on celery seedlings is still unclear. In this study, we explored the effects of different concentrations of sodium hypochlorite solution on the soluble sugar content of celery seedlings at three growth stages(20, 40 and 60 days after sowing), and used the RT-qPCR technology to detect and analyze the expression profiles of soluble sugar synthesis related genes(SS, SPS, UGPase, SPP and Ivr). The results showed that soaking seeds with 5% sodium hypochlorite solution can increase the soluble sugar content of celery seedling leaves with the highest contents. Among them, the sugar content in leaves of celery cultivars ‘Luhe Huangxinqin’ and ‘Saixue’ were 13.20 and 14.00 mg·g^(-1)(FW), respectively, at 60 days after sowing. In addition, under the treatment of 5% sodium hypochlorite, the relative expression levels of the soluble sugar synthesis-related genes, SS and SPS, were the highest in the three stages of two cultivars. The results indicated that 5% sodium hypochlorite solution soaking celery seeds was the suitable concentration to increase the soluble sugar content of seedlings, which provided a theoretical reference for improving the production of celery.