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鸡场铜绿假单胞菌外毒素A基因遗传变异分析

Genetic variation analysis of exotoxin A gene of Pseudomonas aeruginosa isolated from chicken farms
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摘要 为探究铜绿假单胞菌(Pseudomonas aeruginosa,PA)毒力因子变异情况,对鸡场分离、鉴定的7株PA分离菌外毒素A(Exotoxin A,ETA)基因进行了遗传变异分析。结果表明,鸡场分离鉴定的7株PA分离菌成熟的ETA结构蛋白基因长1824和1839 bp,编码608和613个氨基酸;7株分离菌ETA基因与27株PA参考菌株之间核苷酸和氨基酸的同源性分别为98.9%~100.0%,98.5%~100.0%。7株分离菌成熟的ETA结构蛋白基因共有多处碱基位点发生了变异,其中包括PA-47株第106~110位15个碱基的连续缺失和10处错义突变。7株PA分离菌ETA蛋白共有6种不同的氨基酸变异位点模式;第426位(H)、第440位(H)、第470位(Y)、第481位(Y)、第553位(E)和第558位(W)氨基酸残基均未发生突变,均为有毒形式。本研究结果可为PA致病机理研究提供参考依据。 In order to explore the variation of virulence factor of Pseudomonas aeruginosa(PA),genetic variation of Exotoxin type A(ETA)gene was analyzed in seven PA strains identified from chicken farms.The results showed that the mature ETA structural proteins of seven PA strains were composed of 608 and 613 amino acids coded by 1824 and 1839 nucleotides.The sequence similarity of ETA nucleotide and amino acid were 98.9%-100.0%and 98.5%-100.0%among seven strains and twenty-seven reference strains.Several mutation sites were found in the seven strains,including continued deletion of 15 nucleotides at position 106-110 and 10 missense mutations in PA-47 strain.The mature ETA protein comprised of 6 different amino acid mutation patterns.Amino acid residues including 426(H),440(H),470(Y),481(Y),553(E)and 558(W)were not mutated,indicating that the ETA proteins of seven PA strains was all toxic.The results of this study provides a reference on the research of PA pathogenesis.
作者 穆英丽 张芳 解佳 李玉荣 刘聚祥 王学静 陈立功 MU Yingli;ZHANG Fang;XIE Jia;LI Yurong;LIU Juxiang;WANG Xuejing;CHEN Ligong(College of Veterinary Medicine,Hebei Agricultural University,Baoding 071001,China;Center for Animal Disease Prevention and Control of Baoding,Baoding 071051,China;North China Research Center of Animal Epidemic Pathogen Biology,Ministry of Agriculture and Rural Affairs,P.R.China,Baoding 071001,China;The Animal Husbandry and Veterinary Institute of Heibei,Baoding 071000,China)
出处 《河北农业大学学报》 CAS CSCD 北大核心 2022年第1期90-96,共7页 Journal of Hebei Agricultural University
基金 河北省高等学校科学技术研究项目(z2015073) 河北省现代农业产业技术体系蛋肉鸡创新团队(HBCT2018150205、HBCT2018150210).
关键词 铜绿假单胞菌 鸡场 外毒素A 序列分析 Pseudomonas aeruginosa chicken farms exotoxin type A sequence analysis
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